General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 OXTR β-Arrestin Cell Line, 2 vials. (93-0261C2)
The PathHunter OXTR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXTR and EA-tagged β-Arrestin-2. Activation of OXTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Ureaplasma parvum
Ureaplasma parvum strain U26 was isolated from a pregnant woman. This strain requires an additional 5-10% horse serum added to the broth for growth.
ATCC DMS 79 ; Small Cell Lung Cancer; Human (Homo sapiens)
DMS 79 is a cell line that was isolated from the pleural effusion of a 65-year-old, White patient with carcinoma. This cell line was deposited by OS Pettengill, G Sorenson, and can be used in cancer research.
Eurofins Discoverx cAMP Hunter CHO-K1 ADRA2C Gi Cell Line, 2 vials. (95-0053C2)
The cAMP Hunter ADRA2C Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2C GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2C, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2C, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Lonza Walkersville HRCE – Human Renal Cortical Epithelial Cells
Lonza's Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type.Human Renal Proximal Tubule Cells are isolated from the proximal tubule and are ideal for drug transporter studies. Human Renal Epithelial Cells (HRE) are comprised of cells from the cortex and glomerular region. Human Renal Cortical Cells (HRCE) are from proximal and distal tubules. Human Mesangial Cells (HMC) are isolated from the renal glomerulus and modified smooth muscle cells between capillaries.Benefits:Guaranteed through 15 population doublings Test negative for mycoplasma, bacteria, yeast, and fungiHIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lotsA Certificate ofAnalysis is provided for each cell lot purchased Content:1 x Cryopreserved ampule of HRCE containing ≥500,000 cells
Eurofins Discoverx PathHunter CHO-K1 mNPY2R β-Arrestin Cell Line, 2 vials. (93-0569C2)
The PathHunter mNPY2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. peptide YY) based activation of mNPY2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mNPY2R and EA-tagged β-Arrestin-2. Activation of mNPY2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Dermal Fibroblasts; Normal, Human, Neonatal
Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn) is a cell line isolated from neonatal foreskin that has research applications in responding to pathogens, skin aging, wound healing, gene delivery, and skin diseases, including scleroderma.
Eurofins Discoverx ChemiScreen Motilin Receptor Stable Cell Line, 2 vials. (HTS121C)
The ChemiScreen Motilin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MLNR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MLNR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter CHO-K1 mOPRD1 β-Arrestin Cell Line, 2 vials. (93-0611C2)
The PathHunter mOPRD1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. DADLE) based activation of mOPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRD1 and EA-tagged β-Arrestin-2. Activation of mOPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section - Human Throat Tumor: Squamous Cell Carcinoma of Pharynx, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Paraffin Tissue Section - Human Pelvic Tumor: Malignant Mesothelioma, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Frozen Tissue Section - Dementia: Brain: Hippocampus, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx PathHunter U2OS TORC3 Nuclear Translocation Cell Line, 2 vials. (93-1120C3)
The PathHunter U2OS TORC3 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the CRTC/CREB pathway. This cell line enables assessment of ligand (e.g. forskolin) based pathway activation via detection of resulting TORC3 (CRTC3) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC3 (CRTC3) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC3 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx PathHunter U2OS EpoR/EpoR Dimerization Cell Line, 2 vials. (93-0965C3)
The PathHunter EpoR/EpoR Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. erythropoietin) induced dimerization of EpoR/EpoR receptors. This product uses EFC technology. The cell line included overexpresses EpoR target protein fused to PK alongside EpoR target protein fused to EA. Upon ligand-induced dimerization of EpoR/EpoR, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Liver, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.