General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter OPRK1 Total Internalization U2OS Cell Line, 2 vials. (93-0828C3)
The PathHunter OPRK1 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of OPRK1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged OPRK1 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRK1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC pVC-604 plasmid; in E. coli
pVC-604 plasmid in E. coli is a vector that can be used with transformation-associated recombination (TAR) in yeast.
Promocell GmbH PromoCell HDBEC-c 500,000 Human Dermal Blood Endothelial Cells, cryopreserved
Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.
Promocell GmbH PromoCell HCAEC-c 500,000 Human Coronary Artery Endothelial Cells, cryopreserved
Primary Human Coronary Artery Endothelial Cells (HCAEC) are isolated from the coronary arteries (the right and the left coronary artery, including the anterior descending and the circumflex branches) from a single donor. They are crucially involved in the regulation of coronary blood flow and cardiac functions and are consequently useful for in vitro studies of diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also suitable for studying functional vaso-dilators, such as prostacyclin, bradykinin, or nitric oxide.
Promocell GmbH PromoCell hMSC-AT Pellet from > 1 million Normal Human Mesenchymal Stem Cells, derived from Adipose Tissue
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
STEMCELL Technologies Human Peripheral Blood-Derived M0 Macrophages, SF-Cultured, Frozen, Size: 1.5 x 10^6 cells
Primary human cells, frozen
ATCC Enterobacter cloacae; Strain 1101152
Enterobacter cloacae strain 1101152 is a whole-genome sequenced bacterium that was isolated from a clinical specimen. This strain demonstrates resistance to ertapenem and imipenem and was confirmed by PCR to contain the blaKPC gene. It can be used in antimicrobial resistance research and multidrug testing.
ATCC CheRiff_eGFP tet-on; spiking HEK cells; Embryonic Kidney; Human (Homo sapiens)
CheRiff_eGFP tet-on spiking HEK cells is a cell line exhibiting epithelial-like morphology that was isolated from embryonic kidney tissue. It has applications for synthetic electrophysiological systems, and is useful in testing channelrhodopsins and voltage indicators.