General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS NIK Signaling Assay, 2 vials. (93-1059C3)
The PathHunter NIK Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD40, TWEAK) induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein. Upon activation of NFκB signaling by a ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PathHunter CHO-K1 GPR15 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0682C2)
The PathHunter GPR15 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR15 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR15 and EA-tagged β-Arrestin-2. Activation of GPR15 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Cell Systems Corporation Primary Human Brain Microvascular Endothelial Cells (ACBRI 376 V)
Primary Human Brain Microvascular Endothelial Cells (ACBRI 376 V) were initiated from normal human brain cortex tissue. Unlike cells from other providers, Cell Systems primary cells are isolated without antibody labels for enhanced biological relevance. Each vial contains ~1 x 10^6 cells with 85-92% viability under ideal conditions. They are available at Passage 3 (<12 cumulative population doublings) and will initiate a Passage 4 cell culture in a 75 cm2 flask. Each vial of cells is shipped with Bac-Off (antibiotic) and CultureBoost (animal derived growth factors) at no additional cost. You can enhance consistency in your research program with reserved lots. Need vials in bulk? Contact your Fisher Scientific Sales representative for tailored quotes. Tight Deadline? Our cells are available for next day delivery after the purchase order is received. Accelerate Your Research! Try our Attachment Factor (4Z0-201, 4Z0-210) – innovative 5 seconds plating solution for all surfaces.
Genscript Corporation CHO-K1/CD16A 158V Stable Cell
Recombinant CHO-K1 cells stably overexpress human low affinity immunoglobulin gamma Fc region receptor III-A (Fc RIIIa/CD16A 158V) and Fc epsilon receptor Ig (FcER1G) on the surface The surface expression of CD16A 158V is validated by FACS analysis
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Vagina, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
ATCC SK-N-FI; Neuroblastoma; Human (Homo sapiens)
SK-N-FI are neuroblasts that were isolated from the brain of an 11-year-old, White, male patient with neuroblastoma. It has applications in immunology and neuroscience.
ATCC Streptococcus agalactiae
Streptococcus agalactiae strain NCTC 8181 [G19] is a whole genome-sequenced strain that has applications in quality control and bioinformatics. This strain is non-hemolytic and is typed as Lancefield’s group B.
Eurofins Discoverx PathHunter HEK 293 P2RY4 β-Arrestin Cell Line, 2 vials. (93-0263C1)
The PathHunter P2RY4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. UTP) based activation of P2RY4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY4 and EA-tagged β-Arrestin-2. Activation of P2RY4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Fusobacterium nucleatum; Strain VPI 4355; genomic DNA
Genomic DNA from Fusobacterium nucleatum subsp. nucleatum strain VPI 4355 (ATCC 25586). This whole-genome sequenced product can be used in PCR or other molecular biology applications.
iXCells Biotechnologies Human Hepatic Sinusoidal Endothelial Cells (HHSEC, Adult)
iXCells Biotechnologies provides high quality HHSEC, which are isolated from normal human liver and cryopreserved at P2, with >0.5 million cells in each vial. HHSEC express vWF/Factor VIII and CD31. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Endothelial Cell Growth Medium under the condition suggested by iXCells Biotechnologies.
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Heart: Ventricle (right), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biolegend PE anti-mouse CD24 Recombinant
PE anti-mouse CD24 Recombinant [QA20A91]; Isotype: Mouse IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 25 ug
Biolegend TotalSeqTM-C0242 anti-human CD
TotalSeq™-C0242 anti-human CD192 (CCR2) [K036C2]; Isotype: Mouse IgG2a, κ; Reactivity: Human; Apps: PG; Size: 10 μg
Eurofins Discoverx ChemiScreen CCR4 Receptor Stable Cell Line, 2 vials. (HTS009C)
The ChemiScreen CCR4 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. TARC, MDC) based activation of CCR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter CHO-K1 mPTAFR β-Arrestin Cell Line, 2 vials. (93-0614C2)
The PathHunter mPTAFR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAF) based activation of mPTAFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTAFR and EA-tagged β-Arrestin-2. Activation of mPTAFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.