General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 NPR1 Functional Assay, 2 vials. (93-0804C2)
The PathHunter CHO-K1 NPR1 Functional Assay is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting NPR1. This cell line enables assessment of ligand (e.g. ANP) based stimulation of receptor activity via detection of resulting increases in the levels of intracellular cGMP. This product uses EFC technology. The included cell line overexpresses untagged NPR1 Upon activation of the pathway, the increase in levels of intracellular cGMP leads to production of increased EFC signal when the cGMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter PTAFR Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0680C3)
The PathHunter PTAFR Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of PTAFR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTAFR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTAFR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Blastocystis hominis; JDR
Blastocystis hominis strain JDR is a parasitic protozoan that was isolated from a human with diarrhea in Bethesda, Maryland. This product has applications in enteric disease research.
Biochain Institute Inc Multiple Tumor Tissue Array - Bladder, Colon, Kidney, Lung, Stomach, and Uterus tumors, 2 slides/PK
BioChain's tissue arrays or tissue microarrays (TMAs) are a useful tool in biomarker research and drug target validation. It consists of paraffin blocks that are assembled in array fashion to allow multiplex histological analysis. BioChain's TMAs provide a valuable tool for analysis of protein, RNA, or DNA molecules in oncology, both the malignant and metastatic markers. TMAs can also be constructed with tissues from other diseases and species.
ATCC Pseudomonas fluorescens; phage phi-s1
Pseudomonas fluorescens bacteriophage phi-S1 was isolated from sewage. This bacteriophage is propagated in Pseudomonas fluorescens strain PW (ATCC 27663).
Eurofins Discoverx PrecisION hASIC3 Recombinant Stable Cell Line, 2 vials. (CYL3055)
The PrecisION hASIC3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying ASIC3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses ASIC3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Discovery Life Sciences Ulcerative Colitis PBMC
PBMCs Peripheral Blood Mononuclear Cells are immune cells isolated from whole blood and viably cryopreserved providing a foundational material for immunology studies cell therapy research and functional immune assays
Eurofins Discoverx KILR SKOV3 Cell Pool, 2 vials. (97-1007P023)
The KILR SKOV3 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of SKOV3 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Genscript Corporation CHO-K1/ADRA2B/Gqi5 Stable Cell
Adrenoceptors are 7-transmembrane receptors which mediate the central and peripheral actions of the neurotransmitter noradrenaline (norepinephrine) and the hormone and neurotransmitter adrenaline (epinephrine) Based on both pharmacological and molecular evidence they are divided into three major types - 1 2 and The 2 adrenoceptors include 3 subtypes - 2A 2B and 2C - each of which couples to G sub i/o /sub protein primarily br The 2B adrenoceptors (ADRA2B) is expressed in the kidney liver brain lung heart and skeletal muscle The development of 2B and 2C knock-out mice has shown that these two subtypes are not involved in the central hypotensive response to 2 agonists
ATCC Sendai virus; Strain: Cantell
Sendai virus strain Cantell is propagated in SPF chicken embryonated eggs. This strain was deposited by the Central Public Health Laboratory, Helsinki, Finland. This product is whole-genome sequenced and is widely used for induction of interferon and cell fusion. It also has applications in infectious and respiratory disease research.
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099LRTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Promocell GmbH PromoCell HDMEC-c 500,000 Human Dermal Microvascular Endothelial Cells, cryopreserved
Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of juvenile foreskin and adult skin (different locations). Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.In addition, HDMEC are available which are pre-screened for VEGF (vascular endothelial growth factor) response. VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis, cell migration, sprouting, and microvascular permeability.
Eurofins Discoverx KILR SK-MEL-28 Cell Pool, 2 vials. (97-1017P031)
The KILR SK-MEL-28 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of SK-MEL-28 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.