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Normal sera are used to counteract the non-specific interaction between the sample and immunoglobulins. They can be added to the blocking solution and the incubation media. As a rule the normal serum species should be the same as the secondary antibody species (use normal goat serum with goat-anti-rabbit conjugates). Note: normal sera should not be used in combination with Protein A and Protein G gold reagents.
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Horse serum produced from adult horses is homogenous and has low levels of polyamine oxidase. It is metabolically stable and promotes cell proliferation. Horse sera is an alternate to bovine serum and is a source of growth factors and hormones.
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Conventional ImmunoGold Reagents are monodisperse size population makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is abundant and the accessibility of the antigen is relatively good.
The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. The particle population is monodisperse and thus shows minimal size variation and overlap.
The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3. Particle size: EM-grade 10 nm
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