Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,180)
Mouse Secondary Antibodies
(14,490)
Rabbit Secondary Antibodies
(6,301)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(76)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Rabbit anti-Mouse IgG1 Secondary Antibody (20) [DyLight 550], Novus Biologicals™
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Rabbit Monoclonal Antibody
Rabbit anti-Human IgG1 Fc Secondary Antibody (245), DyLight 594, Novus Biologicals™
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Rabbit Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | DyLight 594 |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG1 Fc |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human IgG1 Fc (Uniprot#: P01857-1; Glu99-Lys330,C103S). HEK293 used as expression system. |
| Primary or Secondary | Secondary |
| Clone | 245 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody has been used in 2 publications
| Content And Storage | Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Immunomicroscopy,Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin, Immunomicroscopy |
| Formulation | 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. with 0.1% Kathon |
| Classification | Polyclonal |
| Immunogen | Purified Rabbit IgG (H&L) |
| Reconstitution | Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates. The 0.1 mg size will be shipped in liquid. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, human, mouse IgG serum proteins. |
MilliporeSigma™ Mouse anti-Human IgG1, Fc Fragment Specific (HP6070), Clone: HP6070, Secondary Antibody,
Mouse Monoclonal Antibody
| Antigen | IgG1, Fc Fragment Specific (HP6070) |
|---|---|
| Regulatory Status | RUO |
| Content And Storage | −20°C |
| Host Species | Mouse |
| Applications | ELISA |
| Form | Diluted |
| Formulation | Aschites diluted in PBS, pH 7.4. |
| Classification | Monoclonal |
| Isotype | IgG1 |
| Primary or Secondary | Secondary |
| Clone | HP6070 |
Mouse anti-Rat IgM Heavy Chain Secondary Antibody (HIS40), Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rat |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | Flow Cytometry,Immunohistochemistry,Immunohistochemistry (Paraffin) |
| Isotype | IgG1 κ |
| Research Discipline | Immunology |
| Concentration | 0.5 mg/mL |
| Antigen | IgM Heavy Chain |
| Gene Symbols | IGHM |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Monoclonal |
| Immunogen | Rat IgM |
| Primary or Secondary | Secondary |
| Test Specificity | This secondary antibody eacts with the mu heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugate is used as a detection secondary for rat IgM primary antibodies. |
| Clone | HIS40 |
Mouse anti-Human IgE Heavy Chain Secondary Antibody (E454 (5D5)), FITC, Novus Biologicals™
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Mouse Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | FITC |
| Target Species | Human |
| Host Species | Mouse |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG2a |
| Antigen | IgE Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Protein G purified |
| Gene Alias | Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon |
| Formulation | PBS |
| Classification | Monoclonal |
| Immunogen | Immunoglobulin E from human serum |
| Primary or Secondary | Secondary |
| Clone | E454 (5D5) |
Goat anti-Rat IgG1 Secondary Antibody, Janelia Fluor 549, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | Janelia Fluor 549 |
| Target Species | Rat |
| Host Species | Goat |
| Applications | ELISA,Flow Cytometry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG1 |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Gene Alias | IgG1, Igh-4, RGD1359539, VH7183 |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Rat IgG1 |
| Primary or Secondary | Secondary |
Goat anti-Mouse IgA Secondary Antibody, Janelia Fluor 646, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | Janelia Fluor 646 |
| Target Species | Mouse |
| Host Species | Goat |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgA |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Gene Alias | Immunoglobulin A |
| Formulation | 50mM Sodium Borate |
| Gene ID (Entrez) | 3493 |
| Classification | Polyclonal |
| Immunogen | Mouse IgA paraproteins |
| Primary or Secondary | Secondary |
Goat anti-Mouse IgG F(ab) Secondary Antibody, DyLight 550, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 550 |
| Target Species | Mouse |
| Host Species | Goat |
| Applications | ELISA,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG F(ab) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Mouse IgG Fab fragments |
| Primary or Secondary | Secondary |
Goat anti-Rat IgM (H+L) Secondary Antibody, DyLight 680, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 680 |
| Target Species | Rat |
| Host Species | Goat |
| Applications | ELISA,Flow Cytometry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgM (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Rat IgM |
| Primary or Secondary | Secondary |
Goat anti-Rat IgG2b Secondary Antibody, PerCP, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | PerCP |
| Target Species | Rat |
| Host Species | Goat |
| Applications | ELISA,Flow Cytometry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG2b |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | PBS |
| Classification | Polyclonal |
| Immunogen | Rat IgG2b |
| Primary or Secondary | Secondary |
Goat anti-Human IgD Heavy Chain Secondary Antibody, Janelia Fluor 646, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | Janelia Fluor 646 |
| Target Species | Human |
| Host Species | Goat |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgD Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Gene Alias | Constant Region of Heavy Chain of IgD, Ig Delta Chain C Region, Ig Delta Chain C Region NIG-65, Ig Delta Chain C Region WAH, Immunoglobulin Delta, immunoglobulin heavy constant delta |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Human IgD paraproteins |
| Primary or Secondary | Secondary |
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody, R&D Systems™
Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody, R&D Systems™
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Donkey Polyclonal Antibody has been used in 6 publications
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody, R&D Systems™
Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody, R&D Systems™
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Donkey Polyclonal Antibody has been used in 9 publications
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody, R&D Systems™
Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody, R&D Systems™
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Donkey Polyclonal Antibody