This product is a blue-colored universal SYBR Green reagent, convenient for sampling and using specially designed reference dye (ROX) for higher sensitivity and resolution. It is compatible with all models of fluorescence quantitative PCR instruments (including High ROX, Low ROX, and No ROX required instruments). The reagent introduces a dUTP/UDG system preventing the impact of amplification product contamination on qPCR and ensuring the accuracy of the results. UDG can quickly degrade pollutants containing U at room temperature, and UDG quickly deactivates when pre denatured at 95 ℃, without affecting the efficiency and sensitivity of qPCR. Hot start Taq DNA polymerase is used for amplification, which enhances the specificity of the product. Overall, this product provides a reliable and versatile solution for SYBR Green-based qPCR experiments.