Nucleic Acid Introduction Reagents
Amsbio LLC (formerly Genlantis) GenePORTER™ 2 Transfection Reagents and Kits
Maximum transfection efficiency in hard-to-transfect cells
CTS™ LV-MAX™ Transfection Kit
The Gibco CTS LV-MAX Transfection Kit is an essential component of the CTS LV-MAX lentiviral production system.
Edvotek™ Reagents For Bacterial Transformation: X-gal
For rapid preparation of bacterial cultures for transformation.
Boca Scientific Inc LentiBlast Premium Transduction Enhancer, 1500ul
LentiBlast Premium Transduction Enhancer is designed to increase lentiviral infection and transduction efficiency in a wide range of cell types, from CD34+ hematopoietic stem cells to primary cells or cell lines such as HT1080 or SH SY5Y. LentiBlast Premium is composed of only one reagent, allowing for a new single-step protocol. With LentiBlast Premium, neutralizing electrostatic repulsions between membranes and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable membrane permeable effect limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.
Lonza Walkersville SE Cell Line 96-well Nucleofector Kit (960 RCT)
The SE Cell Line 96-well Nucleofector Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL SE Cell Line Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)10 x 96-well Nucleocuvette Plate (20 μL)
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENE(TM) 9 DNA Transfection Reagent Polymer reagent for transfecting common cell lines |
X-tremeGENE(TM) 9 DNA Transfection Reagent Polymer reagent for transfecting common cell lines |
Lonza Walkersville Cell Line Nucleofector Kit V
The Cell Line Nucleofector Kit V is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b System. This kit has been determined to be optimal for transfecting following cell types:32D3T3-L1pre-adA20A375A7r5AGSARPE-19B16-F10BA/F3 (DSMZ)C2C12C6Capan-1CHO [suspension]CHO-S [suspension] (Life Technologies)COS-1HaCaTHCT116HEK293HepG2HL-60HUV-EC-CJurkatK-562 (DSMZ)MCF7MDA-MB-231MDA-MB-468NB-4 (DSMZ)NCTC clone 929Neuro-2a [N2a]NG108-15P19PC-12PC-3RajiRamosRAW 264.7RBL-1S49Saos-2Schneider‘s Drosophila Line 2SH-SY5YSK-N-SHSK-OV-3SW480T/C-28 a2T/GHA-VSMCT-47DTHP-1U-2 OSVeroBenefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells Low-throughput transfection in single cuvette format
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit S
The P3 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applications Optimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL P3 Primary Cell Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit L
The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel24 x Single use pipettes
Lonza Walkersville SF Cell Line 4D-Nucleofector X Kit S
The SF Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemTransferability to 4D-Nucleofector LV UnitApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL SF Cell Line Nucleofector; Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μL)
Zymo Research Corporation Mix & Go E. coli Transformation Kit & Buffer Set, up to 60 ml
The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108–109 transformants/µg of pUC19 DNA but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Mix & Go preparation method and demonstrate fast transformation kinetics.The procedures are easy. Simply culture the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit S
The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL SE Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0),2 x 16-well Nucleocuvette Strips (20 μL)
Takara Bio Xfect™ Transfection Reagent 300 Rxns
Xfect is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. Transfections can be carried out entirely in the presence of serum. 300 Rxns
Genesee Scientific Corporation jetPEI HTS DNA Transfection Reagent, Low Toxicity, High Throughput Screening Compatible, 5 °C ± 3°C
jetPEI HTS DNA Transfection Reagent is a linear polyethylenimine derivative designed for efficient gene delivery in High Throughput Screening (HTS) applications. It features low toxicity, and compatibility with serum and antibiotics, enhancing experimental flexibility. Its exceptional reproducibility ensures reliable results across experiments, making it an ideal choice for both automated and manual workflows.
Mayflower Bioscience LentiBOOST-P Pharma Grade Lentivirus Transduction Enhancer Solution, 1 mL
LentiBOOSTTM is a highly effective, non-cytotoxic transduction enhancer for preclinical and clinical application of lentiviral vectors.