Transfection Reagents
Lonza Walkersville Cell Line Optimization Nucleofector Kit for Nucleofector Device
The Cell Line Optimization Nucleofector Kit is the ideal tool to determine the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). It enables you to conveniently determine the optimal Nucleofection condition of your cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector Solutions, V and L, each of which should be tested in combination with seven different Nucleofector Programs. It is suitable for 18 optimization reactions. Fine-tuning for optimal results can then be performed together with our Scientific Support Team.Benefits:Efficient transfection of virtually any difficult-to-transfect cell lineSimple and rapid optimization completed within just one experimentApplications:Optimization of Nucleofection conditions of virtually any difficult-to-transfect cell line with the Nucleofector II/2b Device
Expression Systems Expres2 TR (Transfection Reagent), 75 ul (included in BestBac kits)
In collaboration with Expres2ion Biotechnologies, Expression Systems offers Expres2 TR Transfection Reagent, formulated and optimized for insect cell culture. Expres2 TR is a cationic lipid formulation designed for the co-transfection or transfection step in baculovirus vector production.
• Formulation works seamlessly with Transfection Medium
• Optimized for use in insect cell culture
• Consistently high transfection rates Increases virus vector production and transient expression
• In stock for immediate shipment
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
EZ BIOSYSTEMS INC HepG2/C3A Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell) 0.5ml
HepG2/C3A Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HepG2/C3A cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HepG2/C3A cells.
Sigma Aldrich Fine Chemicals Biosciences NeuroPorterTM Transfection
Neuroporter(TM) Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons glial cells and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter(TM) Transfection Kit was designed for difficult-to-transfect primary neurons addressing past problems such as poor cell viability low transfection efficiency and neuro-degeneration.
ATCC CF52.Tr (CRL-6244) Canine Adherent Fibroblast Cell Line, BSL-1
CF52.Tr (CRL-6244) is a canine fibroblast adherent cell line from ATCC's NBL Collection supplied for in vitro research applications. The line is maintained under standard mammalian culture conditions and handled at biosafety level 1.
- Derived from Canis familiaris (dog).
- Fibroblast cell type with adherent growth properties.
- Recommended medium: DMEM with 4 mM L-glutamine and 10% fetal bovine serum.
- Incubation: 37°C in a 5% CO2 atmosphere.
- Biosafety level 1 handling.
- Part of the NBL Collection and fully accessioned for research use.
ATCC NCI-H2085 (CRL-5921) Human Lung Adenocarcinoma (NSCLC) Cell Line
NCI-H2085 (CRL-5921) is a human non-small cell lung adenocarcinoma cell line established from a 45-year-old male donor. It is used in lung cancer research, including cell biology, genomics, and preclinical drug testing.
- Species: Homo sapiens.
- Tissue: Lung.
- Disease: Adenocarcinoma (non-small cell lung cancer).
- Donor sex and age: Male, 45 years.
- Growth medium: ACL-4 base; complete medium requires 5% fetal bovine serum.
- Year of origin: 1988.
- Depositors: AF Gazdar, JD Minna.
Mayflower Bioscience LentiBOOST-P Pharma Grade Lentivirus Transduction Enhancer Solution
LentiBOOSTTM is a highly effective, non-cytotoxic transduction enhancer for preclinical and clinical application of lentiviral vectors. As universally acting (receptor independent) adjuvant it can be applied to a wide range of clinically relevant cell types including CD34+ hematopoietic stem cells (HSCs), primary T cells and NK cells. These unique features make it a promising candidate to improve clinical transduction protocols for ex vivo gene therapies and CAR-T cell therapies.
Lonza Walkersville P2 Primary Cell 4D-Nucleofector X Kit L
The P2 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL P2 Primary Cell Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessel12 x Single use pipettes
Cell Applications Inc Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns
Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns