Transfection Reagents
Sigma Aldrich Fine Chemicals Biosciences DOTAP Chloride | 132172-61-3 | MFCD01310872 | 50mg
DOTAP Chloride | Purity: 98% | Mol Wt: 698.54 | 132172-61-3 | MFCD01310872 | 50mg
Expression Systems Expres2 TR (Transfection Reagent), 75 ul (included in BestBac kits)
In collaboration with Expres2ion Biotechnologies, Expression Systems offers Expres2 TR Transfection Reagent, formulated and optimized for insect cell culture. Expres2 TR is a cationic lipid formulation designed for the co-transfection or transfection step in baculovirus vector production.
• Formulation works seamlessly with Transfection Medium
• Optimized for use in insect cell culture
• Consistently high transfection rates Increases virus vector production and transient expression
• In stock for immediate shipment
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
Agilent Technologies SURE ELECTROCOMP CELLS
SURE Electroporation-Competent Cells. For cloning DNA with secondary structures, > /= 1 x 1010 transformants/microgram. Clone unstable DNA, Increase stability of DNA containing inverted repeats or Z-DNA, Produce high-quality miniprep DNA
Lonza Walkersville Human B Cell Nucleofector Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human B cells.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human B Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Biolegend Dry Human AIM Assay Cocktail K
Dry Human AIM Assay Cocktail Kit; Reactivity: Human; Apps: FC; Size: 5 tests
Agilent Technologies TRANSPACK PACKAGING EXTRACT
Transpack Packaging Extract 100 vials Ideal for recovery of all lambda shuttle vectors Lack ofbeta-gal activity No artifacts affecting mutation data Highest recovery per extract reaction Deficient in McrA McrBC Mrr and McrF
Agilent Technologies XL1-BLUE SUB GRADE COMP CELLS
XL1-Blue Subcloning Grade Competent Cells. For cloning where DNA is not limited, >/=1 x 10^6 transformations/ug. Preparation of high-quality plasmid DNA. Allows blue-white color screening.
Sigma Aldrich Fine Chemicals Biosciences Sequa-brene gelatinous solid | 28728-55-4 | MFCD00133397 | 1VL
Sequa-brene gelatinous solid | 28728-55-4 | MFCD00133397 | 1VL
Agilent Technologies XL10-GOLD ULTRACOMP CELLS
XL10-Gold ultracompetent cells are created for transformation of large DNA molecules with high efficiency. Ideal for constructing plasmid DNA libraries, as they decrease size bias and produce larger, more complex plasmid libraries.
Lonza Walkersville Cell Line Nucleofector Kit T
The Cell Line Nucleofector Kit T is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A431A549Caco-2CHO-K1D1 ORL UVAHT-1080NALM-6 (DSMZ)NRKP815RBL-2H3T84TF-1U-87 MGThe respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution T0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Supply Solutions Roche - X-tremeGENE HP DNA Transf. Reag. 0.4 ml; High-performance polymer reagent for transfecting many cell lines; pkg of 0.4 mL (06366244001) pkg of 1.0mL (06366236001) pkg of 5 x 1 mL (06366546001)
Roche - X-tremeGENE HP DNA Transf. Reag. 0.4 ml; High-performance polymer reagent for transfecting many cell lines; pkg of 0.4 mL (06366244001) pkg of 1.0mL (06366236001) pkg of 5 x 1 mL (06366546001); Hazardous: Y
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 4 TRANSFECTION .2ML
NC2071109 DHARMAFECT 4 TRANSFECTION .2ML
Lonza Walkersville P3 Primary Cell 4D-Nucleofector LV Kit XL
The P3 Primary Cell 4D-Nucleofector LV Kit XL* is suited for larger scale transfection of primary cells in the LV Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the LV Nucleocuvette Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.Applications:For large scale transfection of up to 1 x 10⁹ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 1,1 x LV Nucleocuvette Cartridge2 x 4D-Nucleofector LV Reservoir