Transfection Reagents
Andwin Scientific FectoPRO DNA Transfection Kit
The FectoPRO Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount.
STEMCELL Technologies Resazurin (Sodium Salt), 5 g
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media and further differentiated into cells of all three germ layers (Figure 7).
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Biolegend Dry Human IP Backbone Kit
Dry Human IP Backbone Kit; Reactivity: Human; Apps: FC; Size: 5 tests
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO RED TRANSFECTION INDICAT
NC1655776 SIGLO RED TRANSFECTION INDICAT
Sigma Aldrich Fine Chemicals Biosciences Escort IV Transfection Reagent, 1 mL
Escort IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.
Agilent Technologies XL2-BLUE MRF ULTRACOMP CELLS
XL2-Blue MRF' Ultracompetent Cells deficient in all known E. coli K12 restriction systems. Increase the efficiency of introducing eukaryotic DNA into E. coli, mcrA, mcrCB and mrr mutations, prevent cleavage of methylated DNA.
Agilent Technologies XL1-BLUE ELECTROCOMP CELLS
XL1-Blue Electroporation-Competent Cells are optimal hosts for propagating both lambda and plasmid vectors. They are endA minus cells, Improve the quality of miniprep DNA, allow for color screening and single-strand rescue of phagemid DNA
Agilent Technologies ELECTROTEN-BLUE E.COMP CELLS
ElectroTen-Blue Electroporation-Competent Cells 5 x 0.1mL and pUC18 control plasmid Highest electroporation efficiency (Hee) phenotype use for extrememly demanding cloning= 1 x 1010 transformants/microgram
Agilent Technologies BL21(DE3) PLYSS COMP CELLS
BL21(DE3) pLysS Competent Cell show high-level protein expression, codes for T7 lysozyme and a T7 RNA polymerase inhibitor,provide tighter control of protein expression for expression of toxic proteins,can also be used for non-toxic protien
HORIZON DISCOVERY BIOSCIENCES LIMITED Accell siRNA Delivery Media
Accell siRNA Delivery Media is a serum-free and antibiotic-free media designed for efficient delivery of Accell siRNA into difficult-to-transfect cells without the need for transfection reagents or complex delivery protocols. It contains a proprietary formulation of lipids, proteins and other components that enables passive uptake of siRNA for gene knockdown studies. This media is compatible with both suspension and adherent cell types. It simplifies siRNA delivery, reduces non-specific effects and toxicity and improves gene silencing efficiency.