Transfection Reagents
Lonza Walkersville Human Stem Cell Nucleofector Kit 2
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 2 is one of our two kits suited for efficient transfection of primary human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville P2 Primary Cell 4D-Nucleofector X Kit L
The P2 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL P2 Primary Cell Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessel12 x Single use pipettes
New England Biolabs, Inc. NEB Turbo Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
Chemically competent E. coli competent cells suitable for high efficiency transformation and rapid colony growth.
Lonza Walkersville P2 Primary Cell 4D-Nucleofector X Kit L
The P2 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL P2 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel24 x Single use pipettes
Lonza Walkersville Human Stem Cell Nucleofector Starter Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit. The Human Stem Cell Nucleofector Starter Kit is used to determine which one of our two Nucleofector Kits is best suited for transfecting a specific primary human stem cell clone in Nucleofector 2b Device. It is suitable for 18 optimization reactions.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Andwin Scientific JETPRIME SIZE: 5 X 1.5ML + BUF
Jetprime Size: 5 X 1.5Ml + Buffer^ Jetprime Is A NewPowerful & Polyvalent Transfection Reagent DesignedFor Efficient Delivery Of Dna And Sirna Across ManyAdherent Cell Types. Jetprime Provides HigherTransfection Efficiency, Uses Less Reagent, Is CostEffective, And Delivers Gentle Transfections.
Supply Solutions Roche - X-tremeGENE 9 DNA Transf. Reag. 1.0 ml; Polymer reagent for transfecting common cell lines; pkg 0.4 mL (06365779001) pkg of 1.0 mL (06365787001) pkg of 5 x 1.0 mL (06365809001)
Roche - X-tremeGENE 9 DNA Transf. Reag. 1.0 ml; Polymer reagent for transfecting common cell lines; pkg 0.4 mL (06365779001) pkg of 1.0 mL (06365787001) pkg of 5 x 1.0 mL (06365809001)
Lonza Walkersville Human Monocyte Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human monocytes.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Monocytes0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Optimization Nucleofector Kit for Nucleofector Device
The Cell Line Optimization Nucleofector Kit is the ideal tool to determine the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). It enables you to conveniently determine the optimal Nucleofection condition of your cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector Solutions, V and L, each of which should be tested in combination with seven different Nucleofector Programs. It is suitable for 18 optimization reactions. Fine-tuning for optimal results can then be performed together with our Scientific Support Team.Benefits:Efficient transfection of virtually any difficult-to-transfect cell lineSimple and rapid optimization completed within just one experimentApplications:Optimization of Nucleofection conditions of virtually any difficult-to-transfect cell line with the Nucleofector II/2b Device
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Fibroblasts
In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for fibroblasts.Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 90%Viabilities up to 98%Applications:Kit suited for fibroblasts from different mammalian species and various organs Already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc.
Lonza Walkersville Human B Cell Nucleofector Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human B cells.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human B Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Primary Cell Optimization 4D-Nucleofector X Kit
The Primary Cell Optimization 4D-Nucleofector X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector X Unit.Applications:Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector X UnitContent:0.675 mL Nucleofector Solution P10.675 mL Nucleofector Solution P20.675 mL Nucleofector Solution P30.675 mL Nucleofector Solution P40.675 mL Nucleofector Solution P54 x 0.15 mL Supplement 11 x 0.15 mL Supplement 350 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)6 x 20 µL 16-well Nucleocuvette Strips
SignaGen Laboratories GENMUTE REAGNT JURKAT CELL 1ML
GenMute siRNA Transfection Reagent, Pre-optimized for Transfecting Jurkat Cell.