Transfection Reagents
Sigma Aldrich Fine Chemicals Biosciences Sequa-brene gelatinous solid | 28728-55-4 | MFCD00133397 | 1VL
Sequa-brene gelatinous solid | 28728-55-4 | MFCD00133397 | 1VL
Lonza Walkersville Human Macrophage Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human macrophages.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Macrophages0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT SET 4/REAGENT 1.5ML
DharmaFECT set of 4 transfection reagents, 1.5ml
HORIZON DISCOVERY BIOSCIENCES LIMITED ACCELL CYCLOPHILIN B HM 5NMOL
Accell Cyclophilin B Control Pool HUMAN 5nmol
Sigma Aldrich Fine Chemicals Biosciences 1 VIAL HUH-7D 12 CELL LINE HUM
NC3659956 1 VIAL HUH-7D 12 CELL LINE HUM
Novus Biologicals™ Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 Ligand
Applications: Functional
MilliporeSigma™ Novagen™ BacVector™ 3000 Transfection Kit
Designed for efficient, reliable construction of recombinant baculovirus.
MP Biomedicals™ Activated Penetratin™
Successfully used to internalize covalently attached peptides, oligonucleotides and siRNA.
MilliporeSigma™ Novagen™ NoShift™ Transcription Factor Assay Kit
Detects DNA binding protein in microassay plate based method.
Mirus Bio™ TransIT-PRO™ Transfection Kit
Specifically developed for mammalian protein production in suspension CHO and 293 cells. The PRO Boost Reagent is optional and enhances expression in certain media formulations. Use of TransIT-PRO Transfection Kit eliminates the need for a culture medium change post-transfection.
Mirus Bio™ TransIT™-CHO Transfection Kit
TransIT™-CHO Transfection Kit developed for high transfection efficiency and cell viability in CHO cell types. Does not require medium changes and can be performed in serum-containing medium.
Agilent Technologies MBS MAMMAL TRANSFECTION KIT
Transfection MBS Mammalian Transfection Kit increases the efficiency of transfection by 100-fold or more, depending on the mammalian cell line to be transfected and also the yield of stable transformants