Transcription and Translation Reagents
Trilink Bio Technologies Inc CleanCap Reagent AG
CleanCap Reagent AG is a 5X concentrated capping reagent for 5' end repair of DNA or RNA. It contains an enzyme mix to catalyze the addition of a cap structure to the 5' end of RNA or DNA. The cap consists of a guanine nucleotide connected to the 5' end via a 5'-5' triphosphate linkage. This cap protects the RNA or DNA from degradation and is required for efficient translation. The reagent also contains alkaline phosphatase to remove the 5' phosphate left by T4 PNK treatment. CleanCap Reagent AG is optimized for use in common molecular biology applications including library preparation for next generation sequencing.
Clontech Labs 3P Guide-it Long ssDNA Production System v2 | 50 rxns
The Guide-it Long ssDNA Production System v2 enables on-demand production of long single-stranded DNA (ssDNA) up to 5,000 nt in length for use as a repair template in knockin experiments involving CRISPR/Cas9 technology or other genome editing tools. This kit provides a simple and fast method for converting a dsDNA PCR product of any sequence into ssDNA via selective digestion of either the sense or the antisense strand. Each kit allows for preparation of 50 ssDNA templates.
BIOSEARCH TECHNOLOGIES INC (Lucigen) TransforMax Ec100D pir+ Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Apexbio Technology LLC CO-TRA ARCA 5MCTP ΨUTP T7 25R
CO-TRA ARCA 5MCTP UTP T7 25R APEXBIO TECHNOLOGIES
New England Biolabs, Inc. NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) – 96 reactions
The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries. Please note that this kit, the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible), enables use with your own barcode system. This kit contains adaptors but does not include multiplex primers.
New England Biolabs, Inc. NEBExpress Cell-Free E. coli Protein Synthesis System – 100 reactions
The NEBExpress Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.
New England Biolabs, Inc. HiScribe T7 mRNA Kit with CleanCap Reagent AG
The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield. By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail mRNAs can be transcribed with a 5-m7G Cap-1 structure that is polyadenylated translationally competent and able to evade the cellular innate immune response. The kit is formatted with individual vials of NTPs and CleanCap Reagent AG to allow for partial or complete substitution of modified NTPs with a total kit yield of 1.8 mg of mRNA. Cap-1 mRNA synthesized from this kit is suitable for many applications including transfections microinjections in vitro translation preclinical mRNA therapeutic mRNA studies as well as RNA structure and function analysis.
Apexbio Technology LLC EZ Cap™ EGFP mRNA (5-moUTP), 5x1mg (1mg/mL)
This product has EGFP mRNA with Cap 1 structure and contains 5-moUTP modification, which can provide higher transcription efficiency and inhibit RNA-mediated innate immune activation. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC EZ Cap™ mCherry mRNA (5mCTP, UTP), 100ug
mCherry mRNA encodes the red fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sea anemones. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.This mRNA is capped using a co-transcriptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5mCTP/UTP and optimized for mammalian systems. The addition of 5mCTP/UTP and poly(A) tail can reduce host cell immune response and enhance mRNA stability. It mimics a fully processed mature mRNA. Other sizes are also available. Please inqury us for quote.
Bellbrook Labs TRANSCREENER AMP2/GMP2 FP ASSAY
Transcreener AMP2/GMP2 FP Assay (1,000 Assay, 384 Well)
Trilink Bio Technologies Inc ARCA, 1 umole
A key step in cellular mRNA processing is the addition of a 5’ cap structure, which is a 5'-5' triphosphate linkage between the 5' end of the RNA and a guanosine nucleotide. The cap is methylated enzymatically at the N-7 position of the guanosine to form mature mCAP. When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs. If mCAP (pre-methylated CAP) is used, only half of the asymmetrical mCAP will insert in the functional orientation. ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently.