Transcription and Translation Reagents

Transcription and Translation Reagents

A variety of kits, systems, buffers, inhibitors, RNA cap analogs, reagents, and solutions for use in experiments involving transcription and translation. Products can be used in the synthesis of RNA probes.
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1 – 30 of 107 results
1

BD Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit

For measuring expression of the stem cell master regulators'Nanog, Oct3/4, and Sox2'in heterogenous stem cell populations

2

BD Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit

For high purity isolation of hematopoietic progenitor and stem cells (HPCs and HSCs) from bone marrow

3

BD Human and Mouse Pluripotent Stem Cell Analysis Kit

To analyze cells for expression of cell surface and intracellular pluripotency markers and differentiation markers

4

BD Human Induced Pluripotent Stem Cell Analysis and Sorting Kit

For sorting and analysis of induced human pluripotent stem cell (hiPSC) cultures and cells in the process of being reprogrammed

5

BD Stemflow™ Human Pluripotent Stem Cell Sorting and Analysis Kit

Analyzes populations of live and fixed cells for expression of pluripotency markers and differentiation markers and to sort live populations of pluripotent stem cells and their derivatives

6

BD Pharmingen™ Transcription-Factor Buffer Set

Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins

7

BD Human Pluripotent Stem Cell Transcription Factor Analysis Kit

Measuring expression of the stem cell master regulators'Nanog, Oct3/4, and Sox2'in heterogenous stem cell populations

8

Promega Transcend™ Nonradioactive Translation Detection Systems: IRNA

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

9

Promega Wheat Germ Extract

Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) and is used for in vitro translation..

10

Promega TNT™ Coupled Reticulocyte Lysate Systems

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Content And Storage <-65°C
For Use With (Application) GST pull-downs, Gel shift assays, co-immunoprecipitation, characterization of Protein modifications, Protein-RNA interactions, Protein activity studies, confirmation of gene products, Protein truncation test (PTT)
11

Promega Transcend™ Nonradioactive Translation Detection Systems

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

12

Promega RNasin™ Plus RNase Inhibitor

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Content And Storage -30°C to -10°C
Formulation Amount of RNasin Plus RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%
Concentration 40 U/μL
For Use With (Application) RT-PCR, quantitative RT-PCR, in-vitro transcription, in-vitro translation, coupled transcription/translation, useful in any applications where eukaryotic RNase contamination is a potential problem, protection of mRNA in cDNA synthesis reactions
13

Promega T7 RiboMAX™ Express Large Scale RNA Production System

The T7 RiboMAX Express Large Scale RNA Production System is an in vitro transcription system designed to produce milligram amounts of RNA in a short time.

14

Promega T7 RiboMAX™ Express RNAi System

The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce milligram amounts of RNA suitable for use in RNAi assays.

15

Promega TNT™ Coupled Wheat Germ Extract Systems

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Includes 1mL TNT™ Wheat Germ Extract, 90μL TNT™ Reaction Buffer, 60μL TNT™ RNA Polymerase (SP6, T3 or T7, individually or 2 x 30μL when two Polymerases are
No. of Reactions 40
Content And Storage <-65°C
For Use With (Application) Translation of mammalian transcription factors
16

Promega Rabbit Reticulocyte Lysate Systems, Nuclease Treated

Rabbit Reticulocyte Lysate Translation Systems are used in identification of mRNA species, characterization of their protein products and investigation of transcriptional and translational control.

17

R&D Systems™ Mouse Mesenchymal Stem Cell Functional Identification Kit

To verify multipotency of mouse mesenchymal stem cells by in vitro functional differentiation

18

R&D Systems™ Rat Mesenchymal Stem Cell Functional Identification Kit

To verify multipotency of rat mesenchymal stem cells by in vitro functional differentiation

19

R&D Systems™ Human/Mouse/Rat Neural Lineage Functional Identification Kit

To verify the multipotency of neural progenitor cells (NPCs) by in vitro functional differentiation

20

Promega Rabbit Reticulocyte Lysate, Untreated

Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.

21

Promega RiboMAX™ Large Scale RNA Production Systems

Designed for large-scale RNA production, the RiboMAX Systems consistently produce 2-5mg RNA from a 1ml reaction volume.

Includes Linear control DNA, RQ1 RNase-free DNase, Nuclease-free Water, Enzyme mix, rATP, rCTP, rGTP, rUTP, Sodium Acetate, 3m (pH 5.2), 5X Transcription Buffer
Content And Storage -30°C to -10°C
Quantity 1 System
For Use With (Application) Produce 2 to 5mg/mL of RNA in a 1mL reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe™ system transcription reaction
22

Promega E. coli S30 Extract System for Linear Templates

The E. coli S30 Extract System for Linear Templates allows transcription/translation of linear DNA templates.

23

Promega TNT™ T7 Quick Starter Bundle, Colorimetric

Purchase this bundle, and get the TNT T7 Quick Coupled Transcription/Translation System, your choice of Transcend Translation Detection System, and receive two cell-free expression-qualified expression vectors, pTNT and pCMVTNT Vectors, at no extra cost.

24

Promega E. coli T7 S30 Extract System for Circular DNA

The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter.

25

Promega Flexi™ Rabbit Reticulocyte Lysate System

Flexi Rabbit Reticulocyte Lysate System allows optimization of translation reactions for a range of parameters, including Mg(2+) and K(+) and DTT concentration.

26

Promega TNT™ Quick Coupled Transcription/Translation System

The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.

Content And Storage <-65°C
For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
27

Promega TNT™ T7 Quick for PCR DNA

TNT T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum protein expression from PCR templates.

28

Promega Recombinant RNasin™ Ribonuclease Inhibitor

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Content And Storage -30°C to -10°C
Concentration 20 to 40 U/μL
For Use With (Application) RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems
29

Promega RNasin™ Ribonuclease Inhibitor: Natural

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Formulation Amount of RNasin™ RNase inhibitor required to inhibit the activity of 5ng of RNase A by 50%, activity is measured by the inhibition of hydrolysis of Cytidine 2,3'-cyclic Monophosphate by RNase A
Concentration 20 to 40 U/μL
For Use With (Application) RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems, performing RNase-free Antibody staining, enhancement of RNA yields from Riboprobe™ system RNA synthesis reactions
30

Promega Riboprobe™ Systems

The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.

Content And Storage Store at -20°C.
Quantity 1 System
For Use With (Application) Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA