Cell Based Assays
Eurofins Discoverx PathHunter eXpress PRLR-JAK2 Functional Assay, 10-Plate Kit. (93-0687E3CP0L)
The PathHunter PRLR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PRLR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MCHR2 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0691E3CP5L)
The PathHunter MCHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MCHR2 and EA-tagged β-Arrestin-2. Activation of MCHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C Activated GPCR Internalization Assay, 10pl Kit. (93-0669E3CP27L)
The PathHunter HTR2C Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HTR2C via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged HTR2C. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting HTR2C-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXGR1 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0693E2CP1M)
The PathHunter OXGR1 (GPR80, GPR99) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OXGR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXGR1 and EA-tagged β-Arrestin-2. Activation of OXGR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM5 Activated GPCR Internalization Assay, 10pl Kit. (93-0657E3CP5L)
The PathHunter CHRM5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR2 Activated GPCR Internalization Assay, 2pl Kit. (93-0676E2CP0M)
The PathHunter SSTR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of SSTR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR2 Activated GPCR Internalization Assay, 10pl Kit. (93-0677E3CP0L)
The PathHunter TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TACR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR15 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0682E2CP1M)
The PathHunter GPR15 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR15 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR15 and EA-tagged β-Arrestin-2. Activation of GPR15 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM2 Activated GPCR Internalization Assay, 10pl Kit. (93-0656E3CP5L)
The PathHunter CHRM2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR50 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0648E2CP1M)
The PathHunter GPR50 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR50 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR50 and EA-tagged β-Arrestin-2. Activation of GPR50 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM5 Activated GPCR Internalization Assay, 2pl Kit. (93-0657E3CP5M)
The PathHunter CHRM5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cederlane L-Fucose Assay Kit
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Abcam Cholesterol Assay Kit (Cell-Based)
Cholesterol Assay Kit (Cell-Based)
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences Salubrinal (5mg). CAS: 405060-95-9
Cell permeable and selective inhibitor of the phosphatase complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF-2α). Protects ER stress-induced apoptosis (EC50~15µM in PC12 cells stimulated with 750ng/ml of tunicamycin (BML-CC104)). Purity: ≥98% (HPLC(UV)). Appearance: White to off-white powder. Solubility: Soluble in DMSO (300 mg/ml), dioxane (0.5 mg/ml), methanol (5 mg/ml) or acetonitrile (1.5 mg/ml). Long Term Storage: -20°C.
Enzo Life Sciences BAY 61-3606. hydrochloride (1mg). CAS: 648903-57-5
Cell permeable, potent, ATP-competitive and reversible inhibitor of spleen tyrosine kinase (Syk) (IC50=10nM). Highly selective if compared to other kinases; shows no inhibitory effect against Btk, Fyn, Itk, Lyn, and Src up to concentrations of 4.7µM. Anti-inflammatory and orally active. Exhibits good in vivo efficacy in the treatment of various allergy and asthma conditions in rat models. Alternative name: 2-[[7-(3,4-Dimethoxyphenyl)imidazo[1,2-c]pyrimidin-5-yl]amino]pyridine-3-carboxamide . HCl. Purity: ≥98% (HPLC(UV)). Appearance: Yellow solid. Solubility: Soluble in water (30mg/ml), DMSO (2mg/ml) or methanol (1mg/ml). Long Term Storage: -20°C.