Signal Transduction Reagents and Kits
Enzo Life Sciences SB203580, (1mg), CAS Number: 152121-47-6
Protein kinase inhibitor. Cell permeable, specific inhibitor of p38 (SAPK2a). Inhibits p38 MAPK stimulation of MAPKAPK2 (IC.The concentrations of SB203580 required to block Akt phosphorylation (IC50= 3-5µM) are only approximately 10-fold higher than those required to inhibit p38 MAP kinase (IC50=0.3-0.5µM). These data define a new activity for this drug and suggest that extreme caution should be used when interpreting data when SB203580 has been used at concentrations above 1-2µM. Non-specific effects include inhibition of COX-1 and -2. Enhances clonal growth of skin epithelial progenitor cells and stimulates neural stem cell proliferation. Alternative Name: 4-(4-Fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole. Formula: C21H16N3FOS. MW: 377.4. CAS Number: 152121-47-6. Purity: ≥97% (HPLC). Appearance: White to off-white powder. Solubility: Soluble in DMSO (50mg/ml). Long Term Storage: -20°C
Enzo Life Sciences PD-98059, (5mg), CAS Number: 167869-21-8
MEK inhibitor. PD-98059 is a potent and selective inhibitor of MAP kinase kinase (MEK). It selectively blocks the activation of MEK thereby inhibiting the phosphorylation and the activation of MAP kinase. PD-98059 is cell permeable. In PC12 pheochromocytoma cells, it completely blocked the increase in MAP kinase activity produced by NGF, IC50=2µM. Enhances self-renewal of embryonic stem cells PD-98059 is an invaluable tool to help elucidate the role of the MAPK cascade in a variety of biological systems. Inhibits cell growth and proliferation in acute myelogenous leukemia cell lines by causing cell arrest. Formula: C16N13NO3. MW: 267.3. CAS Number: 167869-21-8. Purity: ≥95% (HPLC). Appearance: Pale yellow solid. Solubility: Soluble in DMSO (6.5mg/ml); 25mg/ml in anhydrous DMSO), methanol, dichlormethane and 100% ethanol (0.6mg/ml). Long Term Storage: -20°C
Enzo Life Sciences STA-21, (1mg), CAS Number: 28882-53-3
STAT inhibitor. STA-21 is a novel Stat3-selective inhibitor. It inhibits Stat3 dimerization and DNA binding as well as Stat3-dependent luciferase reporter activity. Alternative Name: (S)-Ochromycinone, Deoxytetrangomycin. Formula: C19H14O4. MW: 306.3. CAS Number: 28882-53-3. Purity: ≥98% (HPLC). Appearance: White to yellow solid. Solubility: Soluble in DMSO (20mg/ml). Use/Stability: Store solutions at -20°C for up to 3 months. Long Term Storage: -20°C
Takara Bio Lenti-X GoStix Plus, 10 Minute quantitative lentiviral titer tests
Lenti-X GoStix Plus; Quantify lentivirus titer in 10 minutes; 50 Tests
Aat Bioquest CALBRYTE-520 ASSAY KIT 1 PLATE
Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit 1 plate
Sigma Aldrich Fine Chemicals Biosciences Oleoyl-L--lysophosphatidic acid sodium salt 98, solid, 22556-62-3, MFCD00133427
Oleoyl-L--lysophosphatidic acid sodium salt 98, solid
Axxora LLC c-diGMP | 61093-23-0 | MFCD31563661 | 1 umol
c-diGMP | Purity: 98% | Mol Wt: 690.4 | 61093-23-0 | MFCD31563661 | 1 umol
Enzo Life Sciences Lysophosphatidic acid, (5mg), CAS Number: 325465-93-8
LPA receptor ligand. Lysophosphatidic acid (LPA) is a multifunctional intercellular phospholipid messenger. LPA stimulates the growth of a variety of cells including fibroblasts, vascular smooth muscle cells, endothelial cells and keratinocytes. It acts as a proliferative and anti-apoptotic factor. Ligand for LPA1 (EDG-2), LPA2 (EDG-4) and LPA3 (EDG-7) receptors. It also inhibits differentiation of neural stem cells into neurons. Alternative Name: 1-Oleoyl-lysophosphatidic acid . sodium salt. Formula: C21H40O7P . Na. MW: 458.5. CAS Number: 325465-93-8. Purity: ≥98% (TLC). Appearance: Waxy solid. Solubility: Soluble in water (5 mg/mL with sonication) or DMSO (0.5 mg/mL warm). Use/Stability: Store as supplied at -20°C for up to 1 year. Store solutions at -20°C for up to 1 month. Long Term Storage: -20°C
Aat Bioquest Cal-590™ AM | 5x50ug
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3 filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM.
Cell Biolabs Inc ViraDuctin™ Retrovirus Transduction Kit, 40 transductions
The rate at which retroviral vectors bind to and infect cells is mostly controlled by diffusion. During retrovirus infection, only a small fraction of the retroviral vectors can transduce target cells. Virion adsorption is the limiting step of this process. The use of polycations, such as Polybrene, is standard in many retroviral infection protocols owing to the observations of improved infection efficiency. Its mechanism of action is thought to involve neutralization of electrostatic repulsion between virion and cell membranes, enhancing attachment. ViraDuctin™ Retrovirus Transduction Kit is a proprietary formulation for the transduction of retrovirus. After forming a complex with retrovirus in culture medium, the ViraDuctin™ reagents typically provide higher transduction efficiency in many cell types compared to reagents such as Polybrene. It is ideal for transduction of nonpermissive cells such as primary cells and stem cells. 40 transductions.
Echelon Biosciences Research Labs PI(4)P DIC8 AMMONIUM 100 ug
Phosphatidylinositol 4-phosphate diC8 (PI(4)P diC8) is a synthetic, purified dioctanoyl PI(4)P.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4-phosphate (PI(4)P) is the biosynthetic precursor to PI(4,5)P2 and has an important roles in regulating sphingomyelin and glycosphingolipid metabolism and membrane trafficking at the exit of the Golgi complex. This item is non returnable
Echelon Biosciences Research Labs GLO-PI(3,4,5)P3BODIPYTMR100ug
BODIPY TMR PI(3,4,5)P3 (BODIPY TMR Phosphatidylinositol 3,4,5-trisphosphate) is a water soluble analog of PI(3,4,5)P3 (PIP3) labeled with the orange fluorophore, Bodipy-TMR, at the sn-1 position.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Montana Molecular Green Downward GENIe cGMP Sensor Kit D0800G, 30mL, CMV
Measure cyclic GMP (cGMP) signaling dynamics and ascertain the specificity of PDE inhibitors or SNP activators with this live cell fluorescent assay. Add GENIe to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates cGMP increase. The assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. Kit includes soluble guanylate cyclase..