The Incucyte 3D Nanowell Plates facilitate high-throughput organoid studies by creating a 3D microenvironment that mimics in vivo conditions supporting organoid growth and differentiation. These plates feature an array of hydrogel-printed microwells each optimized to foster uniform and reproducible organoid formation. Additionally each well contains a dedicated pipetting port for seamless medium changes that can be integrated with automated processes thus enhancing efficiency and reproducibility in large-scale screening assays. Together with the Incucyte Live-Cell Analysis System and Incucyte Organoid Analysis Software Module 9600-0034 these plates enable high-throughput long-term imaging and analysis of organoids providing insights into their growth and viability dynamics. This plate also requires systems to be operating on Incucyte Base Software v2024A or later.