Enzymes and Inhibitors
Cayman Chemical Ac-DEVD-AMC, Purity: ≥95%, CAS: 169332-61-0, 1 mg
A fluorogenic substrate that can be cleaved specifically by caspase-3
New England Biolabs, Inc. T4 PDG (T4 Endonuclease V) – 2000 units
T4 PDG (pyrimidine dimer glycosylase) has both DNA glycosylase and AP lyase activity. The 16 kd protein recognizes cis-syn-cyclobutane pyrimidine dimers caused by UV irradiation. The enzyme cleaves the glycosyl bond of the 5' end of the pyrimidine dimer and the endonucleolytic activity cleaves the phosphodiester bond at the AP site.
Cayman Chemical Ac-DEVD-AMC, Purity: ≥95%, CAS: 169332-61-0, 10 mg
A fluorogenic substrate that can be cleaved specifically by caspase-3
Sigma Aldrich Fine Chemicals Biosciences Heparinase I and III Blend
Heparinase is an inducible non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by flavobacterium heparinum and contains specific sequences of heparin.
Vector Biolabs human EGFR shRNA Adenovirus (Ad-h-EGFR-shRNA)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Abcam Carbo x yumbelliferyl ß-D-galactopyranoside (CUG), 5MG
MW 368.29 Da, Purity >98%. pH dependent blue fluorogenic β-galactosidase substrate that is well retained intracellularly for use in live cell analyses. Below the pKa (7.8), Abs. shifts to shorter wavelengths (325 to 340 nm) and fluorescence intensity decreases. These shifts may be used to measure compartmentalization of intracellular activity. Em = 445nm, ε = 32K is emission of fluorophore 3-carboxyumbelliferone released upon beta-galactosidase enzyme activity.
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. T4 RNA Ligase 2 – 150 units
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity). Unlike T4 RNA Ligase 1 , T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5 phosphate and 3' OH for ligation. The enzyme can also ligate the 3' OH of RNA to the 5 phosphate of DNA in a double stranded structure.
New England Biolabs, Inc. DNase I (RNase-free) – 5000 units
DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5' -phosphorylated and 3' -hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 1250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.