Enzymes and Inhibitors
Invitrogen™ AcTEV™ Protease
Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins
Thermo Scientific™ FastDigest Green Buffer (10X)
Proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes.
Invitrogen™ T4 DNA Ligase Buffer
T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes
Invitrogen™ LR Clonase™ II Plus enzyme
Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.
Thermo Scientific™ Cfr9I (XmaI) (10 U/μL)
The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Thermo Scientific™ SmaI (10 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Thermo Scientific™ ScaI (10 U/μL)
The ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer (Isoschizomers: AssI, BmcAI, ZrmI).
Thermo Scientific™ XhoI (10 U/μL)
Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).
Thermo Scientific™ MlsI (MscI) (5 U/μL)
The MlsI (MscI) restriction enzyme recognizes TGG^CCA sites and cuts best at 37°C in R buffer (Isoschizomers: BalI, MluNI, MscI, Msp20I).
Thermo Scientific™ FastDigest FaqI
Cut at GGGAC(10/14)^ sites with Thermo Scientific™ FastDigest™ BsmFI (FaqI), which performs best using universal FastDigest Buffer (Isoschizomers: BslFI).
Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
Thermo Scientific™ FastDigest Ecl136II
Cut at GAG^CTC sites with Thermo Scientific™ FastDigest™ Ecl136II, which performs best using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ FastDigest ApaI
Cut at GGGCC^C sites with Thermo Scientific™ FastDigest™ ApaI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspOMI).
Thermo Scientific™ AatII (10 U/μL)
Cut at GACGT^C sites with AatII restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ZraI).