Modifying Enzymes
Cayman Chemical N-Oleoyl GlycIn 100mg
An endogenous lipoamino acid; increases triglyceride levels in 3T3-L1 adipocytes, an effect that can be reversed by SR141716; selectively induces the expression of mRNA encoding the CB1 receptor over mRNA encoding the CB2 receptor in the same cells at 50 µM; potentiates glycine-induced chloride currents in Xenopus oocytes expressing α1-, α1β-, α2-, α2β-, or α3 subunit-containing glycine receptors but not those expressing α3β subunit-containing glycine receptors at 1 µM; increases c-Fos protein levels, intracellular calcium levels, and AgRP secretion in mHypoE-38 cells, effects that can be reversed by AM251; reduces binge-like ethanol intake in alcohol-preferring mice without affecting total fluid intake and does not affect sucrose intake in the sucrose preference test in mice at 60 mg/kg; reduces balance impairments, thermal hyperalgesia, mechanical allodynia, aggressiveness, and immobility time in the tail suspension test in a mouse model of traumatic brain injury at 50 mg/kg
Cayman Chemical Oleoyl SerotonIn 10mg
A hybrid molecule patterned after N-arachidonoyl serotonin; inhibits capsaicin-induced TRPV1 channel activation (IC50 = 2.57 μM) without blocking FAAH-mediated hydrolysis of arachidonoyl ethanolamine (IC50 > 50 μM)
AVIDITY LLC BirA biotin-protein ligase bulk lyophilized reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 ug of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.
Worthington Biochemical Corporation CATALASE 10ML
Catalase, Filtered, Minimum Activity: 40000U/mg Protein, Supplied as an aqueous solution of 2X crystallized catalase filtered through a 0.22u pore size Membrane, Store at 2 deg. to 8 deg.C, 10mL
Sigma Aldrich Fine Chemicals Biosciences Butyryl coenzyme A lithium5MG
Lithium Butyryl coenzyme A lithium5MG
Sigma Aldrich Fine Chemicals Biosciences Lipase immoblilized on Imm50G
LIPASE Lipase immoblilized on Imm50G
Zymo Research Corporation Atlantis dsDNase 12.5 U (0.1 U/ul)
Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
Zymo Research Corporation ZymoTaq™ PreMix (50 Rxns)
The inclusion of a heat-activated, thermal-stable DNA polymerase reduces primer dimer and non-specific product formation that can occur when performing conventional PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, realtime and quantitative PCR that are SYBR Green and probe based. The ZymoTaq™ PreMix yields specific amplicon formation with little or no byproducts. Simple and easy to use, just add water, primers, and template DNA to the ZymoTaq™ PreMix and then heat at 95oC for 10 minutes to initiate polymerization.ZymoTaq™ DNA polymerase is a heat-activated, hot start polymerase that has 3-terminal transferase activity. The addition of A overhangs to amplified DNA makes it ideal for use in TA-cloning.
New England Biolabs, Inc. Vaccinia Capping System – 400 units
Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, and translation of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5' terminal triphosphate. Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap 0 structure, m7Gppp5'N to 5' triphosphate RNA. In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM.
New England Biolabs, Inc. Cre Recombinase – 250 units
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused. DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences.
Zymo Research Corporation Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500µl)
The primary yeast lytic activity is -1,3 glucanase and -1,3-glucan laminaripentao-hydrolase. It hydrolyzes glucose polymers at the -1,3-glucan linkages releasing laminaripentaose as the principal product.
New England Biolabs, Inc. cAMP-dependent Protein Kinase (PKA), catalytic subunit – 100000 units
The catalytic subunit of cAMP-dependent Protein Kinase (PKA) is a serine/threonine protein kinase. The recognition motif for phosphorylation by PKA is RRXS/TY, where Y tends to be a hydrophobic residue.
Sigma Aldrich Fine Chemicals Biosciences T4 DNA Ligase recombinant
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3-hydroxyl- and 5-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.ContentsT4 DNA Ligase supplied with 10x concentrated ligation buffer that includes ATP.
Zymo Research Corporation dsDNA Shearase™ Plus 50 U (1 U/ul)
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
New England Biolabs, Inc. T4 Phage beta-glucosyltransferase – 500 units
T4 Phage -glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.