Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white, 12650-88-3, MFCD00131557, 1 g
Lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase. Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Genscript Corporation 3C protease, His, Human
Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Worthington Biochemical Corporation PAPAIN DISSOCIATION SYSTEM
Papain Dissociation System, 100mL of EBBS, 2degree to 8degreeC Storage Temp, 1BX
FUJIFILM BIOSCIENCES INC LYSLY ENDOPEPTIDASE 10AU/VIAL
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase cleaves peptide bonds at the carboxy-terminal of both lysine residues and S-aminoethylcysteine residues, making it a valuable tool for analysis of peptide and protein primary structure and for enzymatic synthesis of Lys-X compounds. Also, it can retain complete activity after incubation in 4M urea or in 0.1% SDS solution. Pkg. Size:1 vial (10 AU), Preparation: A 10AU-vial contains Tris-HCl corresponding to 1mL of 2mm Tris HCl buffer (pH 8.0),MW: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
Worthington Biochemical Corporation Elastase, Lyophilized, 100mg
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. Store at 2-8°C. Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity.
Ankom Technology ALPHA AMYLASE 250ML
Alpha Amylase 250 ml. ANKOM FAA is a starch-hydrolyzing alpha-amylase enzyme with high heat and pH stability derived from a genetically modified strain of Bacillus licheniformis. Used for liquification of starch during Neutral Detergent Fiber Analysis.
Worthington Biochemical Corporation Trypsin, TPCK Treated, 100mg
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)]. Store at 2-8°C. Protect from moisture.
GENOVIS INC Glyserias, 2000 units
Glyserias is an enzyme that cleaves the Fc portion of IgG antibodies. It is produced by Genovis and sold in vials containing 2000 units of the enzyme. Glyserias works optimally at pH 5.5-6.5 and 37°C. It can cleave both native and recombinant IgGs of all subclasses in just 30 minutes. Glyserias enables antibody deglycosylation, antibody fragmentation, and Fc/Fab separation. It has applications in structural biology, effector function studies, mass spec analysis, and antibody drug development. Glyserias provides an easy and gentle method of IgG cleavage compared to traditional proteolytic enzymes.