Modifying Enzymes
New England Biolabs, Inc. Thermolabile Exonuclease I – 3000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
New England Biolabs, Inc. EnGen Cas9 NLS, S. pyogenes – 2000 pmol
EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) ). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.
Sigma Aldrich Fine Chemicals Biosciences Liberase TL Research Grade, 10 mg
Liberase TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
Sigma Aldrich Fine Chemicals Biosciences Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
New England Biolabs, Inc. Rapid™ PNGase F – 50 reactions
Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability ensured for up to 12 months
Takara Bio mRNA Interferase-MazF, 1000 units
MazF is a toxin protein in the toxin-antitoxin module of E. coli. It possesses endoribonuclease activity and specifically cleaves single-stranded RNA at the 5' end of ACA sequences. This enzyme does not cleave double-stranded RNA, double-stranded DNA or single-stranded DNA.
Worthington Biochemical Corporation DNASE 1 100MG
Deoxyribonuclease I, Chromatographically purified, Equivalent to 1X crystallized Dnase, A lyophilized powder with glycine as a stabilizer, Store at 2 to 8degreeC, Pack Size:100mg, Source: Bovine Pancreas, min. activity: 2000U/mgKunitz dry w
New England Biolabs, Inc. Remove-iT PNGase F – 6750 units
Remove-iT PNGase F is a tagged version of PNGase F, optimized for easy removal from a reaction. Remove-iT PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.
LGC GENOMICS LLC (Lucigen) CircLigase II ssDNA Ligase, 1,000 U @ 100 U/LAn ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i.e. circularisation) of ssDNA and ssRNA templates.
CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group
Worthington Biochemical Corporation Papain, Lyophilized, 100mg
Supplied as a lyophilized powder prepared from a two times crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. Store at 2-8°C. Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite.
Accelagen TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
Worthington Biochemical Corporation PAPAIN 100MG
Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. C.A.S.: 9001-73-4
New England Biolabs, Inc. Hi-T4™ DNA Ligase – 100000 units
A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase. Hi-T4 DNA Ligase will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids at temperatures as high as 50C.
Supply Solutions Roche - DNase I; Grade II, from bovine pancreas; pkg of 100 mg
Roche - DNase I; Grade II, from bovine pancreas; pkg of 100 mg
Worthington Biochemical Corporation PDS KIT 5/PK
Papain Dissociation System, Papain Vial, Code: PAP2, Vial reconstituted with 5mL of EBSS or equivalent yields a solution at 20U of papain permL in 1mm L-cysteine with 0.5mm EDTA, Store at 2 deg. to 8 deg.C, 5Vi