Modifying Enzymes
New England Biolabs, Inc. Thermolabile Exonuclease I – 3000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
INTACT GENOMICS INC Endonuclease IV (Nfo) 5000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
Worthington Biochemical Corporation MICROCOCCUS LYSODEIKTICUS LYO
Dried cells. Suitable lysozyme substrate. 5gm. Store at 2-8�C.
Sigma Aldrich Fine Chemicals Biosciences Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 1VL
Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 1VL
Carnabio Usa Inc BTN - AURA(AURKA) / 10UG
BTN-AurA(AURKA), Full-length, Wild type, 1-403(end), NP_940835.1, Expressed in Insect (sf21); 10microg
C LECTA GMBH DENARASE High Salt 100 kU Enzymes & Nucleases
The salt-tolerant enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process at salt concentrations above 150 mM - Purity 98% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution - Storage Temperature -20C - Produced under ISO 9001 standard- Manufactured without the use of antibiotics Triton X-100 and raw materials of animal origin- - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
C LECTA GMBH DENARASE High Salt 25 kU Enzymes & Nucleases
The salt-tolerant enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process at salt concentrations above 150 mM - Purity 98% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution - Storage Temperature -20C - Produced under ISO 9001 standard- Manufactured without the use of antibiotics Triton X-100 and raw materials of animal origin- - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
C LECTA GMBH DENARASE High Salt 500 kU Enzymes & Nucleases
The salt-tolerant enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process at salt concentrations above 150 mM - Purity 98% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution - Storage Temperature -20C - Produced under ISO 9001 standard- Manufactured without the use of antibiotics Triton X-100 and raw materials of animal origin- - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
C LECTA GMBH DENARASE High Salt 1000 kU Enzymes & Nucleases
The salt-tolerant enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process at salt concentrations above 150 mM - Purity 98% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution - Storage Temperature -20C - Produced under ISO 9001 standard- Manufactured without the use of antibiotics Triton X-100 and raw materials of animal origin- - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
Carnabio Usa Inc BTN - EGFR[T790M/L858RÙ/ 10UG
BTN-EGFR[T790M/L858R], Cytoplasmic domain, Wild type, Amino Acid 669-1210(end), NP_005219.2, 66 kDa, Insect (sf21); 10microg
Supply Solutions Liberase™ TL Research Grade, pkg of 10 mg (2x5 mg)
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
Cayman Chemical N-Oleoyl GlycIn 10mg
An endogenous lipoamino acid; increases triglyceride levels in 3T3-L1 adipocytes, an effect that can be reversed by SR141716; selectively induces the expression of mRNA encoding the CB1 receptor over mRNA encoding the CB2 receptor in the same cells at 50 µM; potentiates glycine-induced chloride currents in Xenopus oocytes expressing α1-, α1β-, α2-, α2β-, or α3 subunit-containing glycine receptors but not those expressing α3β subunit-containing glycine receptors at 1 µM; increases c-Fos protein levels, intracellular calcium levels, and AgRP secretion in mHypoE-38 cells, effects that can be reversed by AM251; reduces binge-like ethanol intake in alcohol-preferring mice without affecting total fluid intake and does not affect sucrose intake in the sucrose preference test in mice at 60 mg/kg; reduces balance impairments, thermal hyperalgesia, mechanical allodynia, aggressiveness, and immobility time in the tail suspension test in a mouse model of traumatic brain injury at 50 mg/kg