Modifying Enzymes
Carnabio Usa Inc PDHK3(PDK3)/10UG/FULL-LENGTH
PDHK3(PDK3), Full-length, Wild type, Amino Acid 1-406, NP_005382.1, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc PDHK1(PDK1)/10UG/FULL-LENGTH
PDHK1(PDK1), Full-length, Wild type, Amino Acid 1-436, NP_002601.1, Expressed in Insect (sf21); 10microg
Biotang Inc T4 DNA Ligase Recombinant, Cloning of restriction fragments. Joining linkers and adapters to blunt-ended DNA.
T4 DNA Ligase Recombinant, Cloning of restriction fragments. Joining linkers and adapters to blunt-ended DNA.
ABclonal Technology Taq DNA Ligase
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5 -phosphate and the 3 -hydroxyl of two adjacent DNA strands. The target strands need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37°C-75°C). It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.
ABclonal Technology RNase A (Powder)
Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3 end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3 phosphate and an oligonucleotide with pyrimidine 3 phosphate at the end. RNase A does not work on DNA.This product is sourced from bovine pancreas and is supplied in the form of white lyophilized powder with enzyme activity ≥ 50 Kunitz units/mg protein. Its recommended working concentration is 1-100 μg/mL, which will vary depending on specific applications.
Biotang Inc Alkaline Phosphatase Bovine Intestinal, Alkaline Phosphatase Bovine, Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, Mg++ stimulates the catalysis.
Alkaline Phosphatase Bovine Intestinal, Alkaline Phosphatase Bovine, Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, Mg++ stimulates the catalysis.
Amsbio LLC Heparinase I (EC 4.2.2.7) from Flavobacterium heparinum/ 5 IU
Heparinase I (EC 4.2.2.7) from Flavobacterium heparinum 5 IU
ABclonal Technology T4 RNA Ligase 2, truncated KQ
T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q), specifically catalyzes the ligation of a 5-adenylated DNA or RNA to the 3 OH end of an RNA molecule. This enzyme-mediated ligation does not require ATP but necessitates a pre-adenylated adapter. T4 RNA Ligase 2, truncated KQ, a double-point mutant of T4 RNA Ligase 2 with R55K and K227Q substitutions, significantly reduces the non-specific ligation of RNA (such as concatenation or self-circularization) compared to T4 RNA Ligase 2, truncated, while maintaining enzyme activity similar to that of T4 RNA Ligase 2, truncated.
Ubpbio N-terminally 6xHis-tagged recombinant human UbC13/UEV1A (UbE2N), a ubiquitin conjugating enzyme catalyzing formation of K63 polyubiquitin chains
UbE2N (Ubc13/UEV1A) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.
Amsbio LLC Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum/ 0.5 IU
Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum 0.5 IU