Modifying Enzymes
STEMCELL Technologies ArciTect™ T7 Endonuclease I Kit, Size: 25 Reactions
ArciTect™ T7 Endonuclease I is the preferred enzyme for detecting genome editing such as insertions or deletions (INDELs) generated by CRISPR-Cas9. ArciTect™ T7 Endonuclease I Kit is comprised of ArciTect™ T7 Endonuclease I and ArciTect™ T7 Endonuclease I Buffer (10X), which have been tested and validated for use with the ArciTect™ CRISPR-Cas9 genome editing system. ArciTect™ T7 Endonuclease I recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA, and, less efficiently, nicked double-stranded DNA. Since the cleavage efficiency is proportional to the number of INDELs created at a specific DNA target, ArciTect™ T7 Endonuclease I Kit is used to estimate gene-editing efficiency in a rapid and cost-effective manner.
Sigma Aldrich Fine Chemicals Biosciences Lipase acrylic resin >=5,000 U/g, recombinant, expressed in Aspergillus niger | 9001-62-1 | MFCD00217536 | 3G
Lipase acrylic resin >=5,000 U/g, recombinant, expressed in Aspergillus niger | 9001-62-1 | MFCD00217536 | 3G
Amsbio LLC Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum/ 0.5 IU
Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum 0.5 IU
EPICYPHER INC CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
Sigma Aldrich Fine Chemicals Biosciences Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 30ku
Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 30ku
Sigma Aldrich Fine Chemicals Biosciences Malic Dehydrogenase from porcine heart >=600 units/mg protein (biuret), ammonium sulfate suspension | MFCD00131567 | 5KU
Malic Dehydrogenase from porcine heart >=600 units/mg protein (biuret), ammonium sulfate suspension | MFCD00131567 | 5KU
Abcam NADP, Coenzyme, 250MG
MW 787.37 g/mol, Purity >=93%. NADP and NADPH form a redox pair. NADP is the oxidised form of NADPH. NADP/NADPH is a coenzyme that supports redox reactions via the transport of electrons in a vast array of applications. Acts as a coenzyme couple in various cytochrome P450 systems and oxidase/reductase reaction systems.
The product is subject to the following: Abcam Restricted Use Statement
Amsbio LLC Chondroitinase ABC protease free (lyophilized) 2U/vial
Chondroitinase ABC protease free (lyophilized) 2U/vial
ABclonal Technology Endonuclease IV
Endonuclease IV derived from E. coli recognizes the depurine/depyrimidine (AP) site on the double- stranded DNA molecule, and cleaves the first phosphodiester bond at the 5 ?end of the AP site to form 3-OH and 5dRP . In addition, Endonuclease IV has 3?diesterase activity, which releases phosphoglyceraldehyde, intact deoxyribose 5 -phosphate, and phosphoric acid from the 3 ?end of DNA. The best substrate for this enzyme is AP double-stranded DNA, but it is also active against AP single-stranded DNA.
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 25g
Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg proteinPurity: MW: | 12650-88-3 | MFCD00131557 | 25g
Sigma Aldrich Fine Chemicals Biosciences Pectinase from Aspergillus niger BioReagent, suitable for plant cell culture, aqueous glycerol solution, >=5 units/mg protein (Lowry) | 9032-75-1 | MFCD00131809 | 10KU
Pectinase from Aspergillus niger BioReagent, suitable for plant cell culture, aqueous glycerol solution, >=5 units/mg protein (Lowry) | 9032-75-1 | MFCD00131809 | 10KU
ABclonal Technology Mth RNA Ligase
Mth RNA Ligase is an ATP-dependent ligase that can efficiently convert 5-phosphorylated single-stranded DNA into 5-adenylated DNA. The kit has been optimized to yield high quantities of adenylylated DNA regardless of whether the 3 end of the single-stranded DNA has been aminated or otherwise modified. Typically, the modification efficiency can reach over 95%.