Modifying Enzymes
Cayman Chemical Octnoyl-Coenzyme AsodIum s 1mg
A medium-chain acyl CoA; levels increased in liver in patients with Reye’s syndrome and β-oxidation of octanoyl-CoA by MCADH is decreased in patients with MCD; inhibits citrate synthase and glutamate dehydrogenase
Bioworld alpha-Chymotrypsin, 500 mg
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Bioworld alpha-Chymotrypsin, 1 g
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Bioworld Macerozyme R-10, 5 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water
INTACT GENOMICS INC Taq DNA Ligase 10,000 Units
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. This product is active at elevated temperatures (45°C-70°C) (1, 2).Applications Allele-specific gene detection by using Ligase Detection Reaction (LDR) and Ligase Chain Reaction (LCR) (1). Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification(3).
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. Bpu10I – 200 units
A restriction endonuclease that recognizes the sequence CC^TNA_GC.
Agilent Technologies CLONED PFU DNA POLYM
Cloned Pfu DNA Polymerase is a recombinant version for high-fidelity PCR and primer-extension reactions. Utilized for PCR cloning and generating blunt-end amplification product. Cloned Pfu DNA polymerase, 10X Cloned Pfu buffer.
New England Biolabs, Inc. Quick Ligation™ Kit – 150 reactions
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25C)
New England Biolabs, Inc. T7 Exonuclease – 1000 units
T7 Exonuclease acts in the 5' to 3' direction, catalyzing the removal of 5' mononucleotides from linear or nicked duplex DNA.
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.