Modifying Enzymes
LGC GENOMICS LLC (Lucigen) RNA 5' Polyphosphatase, 200U @ 20 U/LDephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs.
RNA 5 Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the and phosphates from 5-triphosphorylated RNA (such as primary RNA transcripts): 5 pppNOH 3 5 pNOH 3 + 2 Pi
STEMCELL Technologies DNase I, ACF, Size: 100000 U
Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography. DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Sigma Aldrich Fine Chemicals Biosciences Collagenase A from Clostridium histolyticum |
Collagenase A from Clostridium histolyticum |
New England Biolabs, Inc. PNGase F – 75000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- Leaves N-glycan core oligosaccharides intact and suitable for further analysis
- Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Stored in 50% glycerol
- Optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
New England Biolabs, Inc. Enterokinase, light chain – 2560 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. Factor Xa Protease – 250 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
New England Biolabs, Inc. Endoproteinase GluC – 50 µg
Endoproteinase GluC is a serine proteinase which preferentially cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues at a rate 100-300 times slower than at glutamic acid residues.
New England Biolabs, Inc. EarI – 2500 units
A restriction endonuclease that recognizes the sequence CTCTTCN^NNN_.
New England Biolabs, Inc. EnGen Cas9 NLS, S. pyogenes – 2000 pmol
EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) ). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.
Qiagen Beverly Inc T3 DNA LIGASE
DNA Ligase; T3 DNA Ligase; Conc. 3,000,000 U/ml; 900,000 U; incl. 2X rapid ligation buffer
AnaSpec ADAMTS-4/Aggrecanase FRET Substrate, FRET Assay, 1 mg
The ADAMTS-4/Aggrecanase FRET Substrate offers researchers a reliable tool for detecting ADAMTS-4 enzyme activity. With a quantity of 1 mg, this peptide substrate enables precise enzyme assays with FRET technology, distinguishing it from standard substrates. Its specific function in cartilage degradation pathways makes it vital for studies related to osteoarthritis and tissue remodeling. Easy to use and store, it supports ongoing research in enzyme substrates and inhibitors.
Medchemexpress LLC α-Amylase-IN-3 | 93944-58-2 | 99.5% | 267.24 | 25 MG
α-Amylase-IN-3 is a chemical compound functioning as an inhibitor for α-Amylase, AChE, and BChE. It also demonstrates antioxidant activity and is suitable for research on diabetes and oxidative stress-associated diseases. This product is intended for laboratory use only.
- Inhibits α-Amylase, AChE, and BChE with specific IC50 values.
- Demonstrates antioxidant activity.
- Applicable in research for diabetes and oxidative stress-associated diseases.
- Designed for laboratory chemical applications.
- Suitable for experienced research personnel.
Indofine Chemical Proteinase K, From Tr, 25 Mg
PROTEINASE K, FROM TRITIRACHIUM ALBUM 25 MG, Products for Life Science, Endopeptidase K, 39450-01-6, MFCD00132129
Medchemexpress LLC HY-19801 5mg Medchemexpress, CVT-10216 CAS:1005334-57-5 Purity:>98%
Medchemexpress, HY-19801 5mg CVT-10216 CAS:1005334-57-5 CVT-10216 is a highly selective, reversible aldehyde dehydrogenase-2 (ALDH-2) inhibitor with an IC50 of 29 nM. CVT-10216 also has inhibitory effect of ALDH-1 with an IC50 of 1.3 μM. CVT-10216 can reduce excessive alcohol drinking in alcohol-preferring rats and exhibit anxiolytic effects. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.