Modifying Enzymes
Enzyme Research Laboratories Inc HUMAN FIBRINOGEN 1G
Human Fibrinogen that is plasminogen, vWF and Fibronectin depleted.
Discovery Life Sciences HUM CYP2E1+REDUCTASE+B5 1NMOLE
Human CYP2E1 + P450 Reductase + Cytochrome b5 SUPERSOMES 1.0 nmole cytochrome P450 in 0.5mL This activity is catalyzed by CYP2E1 which is expressed from human CYP2E1 cDNA using a baculovirus expression system
Enzyme Research Laboratories Inc HUMAN FACTOR ALPHA XIIA 0.5MG
Human Factor alpha -XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.
Discovery Life Sciences HUM CYP2B6+REDUCTASE+B5 0.5NMO
Human CYP2B6 + P450 Reductase + Cytochromebeta5 SUPERSOMES 0.5 nmole cytochrome P450 in 0.5mL 7-Ethoxy-4-trifluoromethylcoumarin deethylase activity has replaced 7-ethoxycoumarin deethylase activity for this enzyme
ABclonal Technology Exonuclease VII
Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5-3 and 3-5 directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzymes digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).
Enzo Life Sciences Cathepsin B (human liver), (purified) (25 µg)
Highly pure lysosomal protease. MW: ~27.5 kDa. Purity: ≥95% (SDS-PAGE). Formulation: Liquid. In 50mM sodium acetate, pH 5.0, containing 1mM EDTA. Source: Purified from human liver. UniProt: P07858. Long Term Storage: -80°C
ABclonal Technology Exonuclease I (E. coli)
Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA in the 3 to 5 direction, and does not degrade DNA strands with the 3-OH end blocked by phosphoryl or acetyl groups. Exonuclease I has strict substrate specificity and is incapable of degrading double-stranded DNA and RNA.
ABclonal Technology T7 Endonuclease I
T7 Endonuclease I (T7 Endo I, T7EI)，can recognize and cleave non-perfectly mismatched DNA, cruciform DNA structures, holliday structures or junctions and Heteroduplex DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5? end to the mismatch.
ABclonal Technology T3 DNA Ligase
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold). As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.
ABclonal Technology DNase I , RNase-free (5,000 U/mL)
DNase I （deoxyribonuclease, RNase-free ） is an endonuclease that nonspecifically cleaves DNA to release di-, tri-, and oligonucleotide products with 5phosphorylated and 3?-hydroxylated ends. The activity of DNase I depends on Ca2+ and also be activated bydivalent metal ions Mg2+, Mn2+, etc.DNase I act on various DNAs such as single and double-stranded DNA, RNA:DNA hybrids.
ABclonal Technology Mth RNA Ligase
Mth RNA Ligase is an ATP-dependent ligase that can efficiently convert 5-phosphorylated single-stranded DNA into 5-adenylated DNA. The kit has been optimized to yield high quantities of adenylylated DNA regardless of whether the 3 end of the single-stranded DNA has been aminated or otherwise modified. Typically, the modification efficiency can reach over 95%.
Discovery Life Sciences HUMAN P450 OXIDOREDUCTASE+B5
BD Gentest, Human P450 Oxidoreductase + b5 Insect Control, Regulatory Status is RUO
Sigma Aldrich Fine Chemicals Biosciences ISOBUTYRYL COENZYME A LITHIUM
NC3840085 ISOBUTYRYL COENZYME A LITHIUM
Sigma Aldrich Fine Chemicals Biosciences Protease from Bacillus licheniformis >=2.4 U/g | 9014-01-1 | MFCD00132092 | 50ML
Protease from Bacillus licheniformis >=2.4 U/g | 9014-01-1 | MFCD00132092 | 50ML