Modifying Enzymes
Macherey-Nagel Liquid RNase A, 100 mg/mL Concentration, Ready-to-Use Liquid, 2.5 mL
MACHEREY-NAGEL Liquid RNase A is a ready-to-use enzyme solution designed for RNA digestion in molecular biology. This highly concentrated RNase A facilitates efficient sample preparation for cell lysis and nucleic acid digestion, eliminating the need for prior dissolution. Its compatibility with NucleoSpin™ and NucleoBond™ kits ensures streamlined workflows. With a concentration of 100 mg/mL and a shelf life of 24 months, it stands out for its quality and effectiveness.
STEMCELL Technologies DNase I, Size: 100 mg
Deoxyribonuclease I (DNase I) is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Teknova qPCR Proteinase K Buffer (1.11M NaCl, 1.11% Sarkosyl). 100mL, Sterile.
1.11M Sodium Chloride,1.11% Sarkosyl
Rockland Immunochemicals Protease S. aureus 1 mg
Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27 000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F- Cl- CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay. Protease S. aureus is ideal for investigators involved in enzyme and infectious diease research.
STEMCELL Technologies ArciTect™ T7 Endonuclease I Kit, Size: 125 Reactions
ArciTect™ T7 Endonuclease I is the preferred enzyme for detecting genome editing such as insertions or deletions (INDELs) generated by CRISPR-Cas9. ArciTect™ T7 Endonuclease I Kit is comprised of ArciTect™ T7 Endonuclease I and ArciTect™ T7 Endonuclease I Buffer (10X), which have been tested and validated for use with the ArciTect™ CRISPR-Cas9 genome editing system. ArciTect™ T7 Endonuclease I recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA, and, less efficiently, nicked double-stranded DNA. Since the cleavage efficiency is proportional to the number of INDELs created at a specific DNA target, ArciTect™ T7 Endonuclease I Kit is used to estimate gene-editing efficiency in a rapid and cost-effective manner.
ILEX LIFE SCIENCES LLC Ilex Life Sciences L-Asparaginase, E. coli Enzyme, Bioactive, 500 IU
Our high-purity, bioactive L-Asparaginase enzyme is purified from E. coli ASI.357. Purity: greater than 96%. Formulation: Lyophilized with no additives. Summary: L-Asparaginase is an enzyme that depletes L-Asparagine, an important nutrient for cancer cells, resulting in cancer/tumor cell starvation. The rationale behind asparaginase is that it takes advantage of the fact that acute lymphoblastic leukemia cells and some other suspected tumor cells are unable to synthesize the non-essential amino acid asparagine, whereas normal cells are able to make their own asparagine; thus leukemic cells depend on circulating asparagine. Asparaginase, however, catalyzes the conversion of L-asparagine to aspartic acid and ammonia. This deprives the leukemic cell of circulating asparagine, which leads to cell death. Synonyms: L-ASNase, L-asparagine amidohydrolase, ansA.
Gold Biotechnology Inc Proteinase K Solution
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins It cleaves at the carboxylic acid side of aliphatic aromatic or hydrophobic amino acids Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids Proteinase K is also useful for helping to characterize enzymes due to its cleavage specificity This enzyme was designated proteinase K because of its ability to hydrolyze keratin Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH The isoelectric point of proteinase K is 8 9
Worthington Biochemical Corporation Collagenase, Type 3, Filtered, 50mg
Collagenase, Type 3 (Code: CLS-3), which is filtered through a 0.22 micron membrane and lyophilized in vials to contain 50 milligrams per vial. Store at 2-8°C.
Worthington Biochemical Corporation DNASE 11MG FILTER
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. PspXI – 200 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.
Supply Solutions Roche - RNA Polymerase, T7, 1000 U; from Escherichia coli BL 21/pAR 1219; pkg of 1,000 U (10881767001) pkg of 5,000 U (10881775001)
Roche - RNA Polymerase, T7, 1000 U; from Escherichia coli BL 21/pAR 1219; pkg of 1,000 U (10881767001) pkg of 5,000 U (10881775001)