Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Phosphodiesterase I from Crotalus adamanteus venom vial of >=100 units, Purified | 9025-82-5 | MFCD00131883 | 1VL
Phosphodiesterase I from Crotalus adamanteus venom vial of >=100 units, Purified | 9025-82-5 | MFCD00131883 | 1VL
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album 3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 1g
Proteinase K from Tritirachium album 3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 1g
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 25g
Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg proteinPurity: MW: | 12650-88-3 | MFCD00131557 | 25g
Sigma Aldrich Fine Chemicals Biosciences Phospholipase C from Clostridium perfringens (C. welchii) Type I, lyophilized powder, 10-50 units/mg protein | 9001-86-9 | MFCD00131895 | 125UN
Phospholipase C from Clostridium perfringens (C. welchii) Type I, lyophilized powder, 10-50 units/mg protein | 9001-86-9 | MFCD00131895 | 125UN
Sigma Aldrich Fine Chemicals Biosciences Lipase from Pseudomonas cepacia powder, light beige, >=30 U/mg | 9001-62-1 | MFCD00131509 | 100MG
Lipase from Pseudomonas cepacia powder, light beige, >=30 U/mg | 9001-62-1 | MFCD00131509 | 100MG
Sigma Aldrich Fine Chemicals Biosciences Papain from papaya latex | 9001-73-4 | MFCD00131791 | 25mg
Papain from papaya latex | Mol Wt: 23.4 kDa | 9001-73-4 | MFCD00131791 | 25mg
Sigma Aldrich Fine Chemicals Biosciences Pectinase from Aspergillus niger BioReagent, suitable for plant cell culture, aqueous glycerol solution, >=5 units/mg protein (Lowry) | 9032-75-1 | MFCD00131809 | 10KU
Pectinase from Aspergillus niger BioReagent, suitable for plant cell culture, aqueous glycerol solution, >=5 units/mg protein (Lowry) | 9032-75-1 | MFCD00131809 | 10KU
Worthington Biochemical Corporation PAPAIN LYOPHILIZED 1GM
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. 9001-73-4
Worthington Biochemical Corporation ELASTASE LYOPHILIZED 1GM
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. 2594-83-7
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I RN 100UN
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1 mM calcium chloride. 9003-98-9
New England Biolabs, Inc. Bacteroides Heparinase III – 14 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
New England Biolabs, Inc. T7 DNA Ligase – 750000 units
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase. However, unlike T4 and T3 DNA Ligases, blunt end ligation is not efficiently catalyzed by T7 DNA Ligase. Addition of high concentrations of PEG 6000 [>= 20% (w/v)] to the reaction can force T7 DNA Ligase to have measurable activity. However, under typical reaction conditions blunt-end DNA ligation does not occur in the presence of T7 DNA Ligase, making it a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined. T7 DNA Ligase does not ligate blunt-end DNA fragments.
New England Biolabs, Inc. Bacteroides Heparinase I – 240 units
Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Worthington Biochemical Corporation STEMXYME 1 COLLAGENAS 50MG
A specialized combination of Animal Origin-Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal origin free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented. 2593-92-3
New England Biolabs, Inc. PNGase F, Recombinant – 15000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F, Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.