Catachem PLASMA FREE HEMO REAG KIT
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For In Vitro Diagnostic Use In The Quantitative Determination Of Plasma Free Hemoglobin In Serum Using Manual Or Automated Applications. Method Principle The Catachem Colorimetric Procedure For The Determination Of Hemoglobin In Plasma Is Based Upon The Peroxidase Activity Of Hemoglobin. In This Procedure, Hemoglobin Activates The Oxidation Of 3.3',5.5'-Tetramethylbenzidine By Hydrogen Peroxide To Form A Chromogenic Product With Maximum Absorption At 600 Nm. The Increase Of Absorbance Is Directly Proportional To The Concentration Of Hemoglobin In The Plasma Sample. This Procedure Is Simple And Accurate And It Is Based On The Work Of Lijana, Rc, And Williams, Mc, And Standefer, J.C. And Vanderjagt, D. Reagent Storage And Stability Catachem Plasma Free Hemoglobin Reagents Are Stored At 2-8�C. When Stored As Directed, The Reagents Are Stable Until Expiration Date Stated On The Label. Working Reagent Preparation Catachem Plasma Free Hemoglobin Reagents Are Packaged In Ready-To-