Hooke Kits™ for EAE Induction in C57BL/6 Mice. Emulsion supplied in pre-filled syringes, ready to use. Consistently induces EAE in female C57BL/6 mice EAE will develop in 90-100% of mice. Disease onset is 9 to 14 days after immunization. Mean maximum score will be 3.0 to 3.5. Each lot is tested and individually adjusted to ensure consistent disease induction. On Day 0, MOG35-55/CFA or MOG1-125/CFA emulsion and pertussis toxin are injected. On Day 1, a second dose of pertussis toxin is injected.roperly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease.
The QuickTiter MuLV Core Antigen ELISA Kit measures the core antigen of MuLV with sensitivity as low as 300 pg/mL. Reagents are sufficient for 96 wells including standards and unknown samples.
EZ-Tn5™ Transposase is a hyperactive Tn5 transposase for random insertion of an EZ-Tn5 Transposon into any cloned DNA in vitro or for preparing an EZ-Tn5 Transposome for random insertion of a EZ-Tn5 Transposon into the genomic DNA of living bacteria.
Trypsin-EDTA for Primary Cells is a low-concentration formulation (0.05% Trypsin and 0.02% EDTA in phosphate buffered saline without calcium or magnesium) of porcine pancreatic trypsin and EDTA that is suitable for the dissociation of cell monolayers that are susceptible to "over-trypsinization." These adherent cells include primary cells (i.e., ATCC Primary Cells Solutions™ cell types) as well as a variety of mammalian cell lines that are propagated in serum-free or low serum conditions. For use by FOR-PROFIT companies only; NON-PROFIT companies use 50189662NP. New ATCC customers are required to register with ATCC prior to purchase. The application can be found at https://www.atcc.org/en/My_Profile/Register.aspx
Trypsin Neutralizing Solution is specifically formulated (5% FBS in phosphate buffered saline without calcium and magnesium) to rapidly inactivate the concentration of trypsin found in the Trypsin-EDTA for Primary Cells solution. For use by FOR-PROFIT companies only; NON-PROFIT companies use 50189663NP. New ATCC customers are required to register with ATCC prior to purchase. The application can be found at https://www.atcc.org/en/My_Profile/Register.aspx
6883S , Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Tacha's Automated Hematoxylin is a water-based hematoxylin, specially formulated for automated IHC. This hematoxylin can be used on FFPE or frozen tissue. Nuclei stain a sky blue, providing high contrast staining.
Normal sera are used to counteract the non-specific interaction between the sample and immunoglobulins. They can be added to the blocking solution and the incubation media. As a rule the normal serum species should be the same as the secondary antibody species (use normal goat serum with goat-anti-rabbit conjugates). Note: normal sera should not be used in combination with Protein A and Protein G gold reagents.
9841S , Bisindolylmaleimide I (BIS) is a potent inhibitor of PKC (1,2). In vitro the IC50 of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The in vitro IC50 for PKCζ is about 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In in vivo cellular assays the IC50 of BIS for PKC is between 0.2-2 μM (1,3).
9844S , H-89 is a potent selective inhibitor of cAMP dependent protein kinase (PKA). The in vitro IC50 of H-89 for PKA is approximately 50 nM and in vivo the inhibitiory effect on PKA substrate phosphorylation and related cellular functions range from 10 μM to 30 μM (1,2). In addition to PKA, H-89 also exhibits a moderate inhibitory effect on PKG and PKCμ, with IC50 in the 500 nM range (1,3). The inhibitory effect of H-89 is due to its competitive binding to the ATP pocket on the kinase catalytic subunit (4).
4410S , This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
3828S , Forskolin, a naturally occurring diterpene from the Indian plant, Coleus forskohlii, activates adenylate cyclase, and thus increases the intracellular cAMP concentration (1). The second messenger cAMP activates cAMP-dependent protein kinase (PKA or cAPK) and controls many cellular mechanisms such as gene transcription, ion transport and protein phosphorylation (2).
The Rat-on-Mouse HRP-Polymer is for use with rat primary antibodies on mouse tissues. This detection system is mouse adsorbed for minimum cross-reactivity to endogenous mouse IgG. It is labeled with horseradish peroxidase (HRP).
Lyophilized assayed chemistry control with method & instrument specific target values and ranges for 70 analytes. Reconstituted Stability of 4 weeks at -20C or 7 days at +2-8C.
MOG35-55/CFA EMULSION PTX 
