Molecular Biology Reagents and Kits
New England Biolabs, Inc. Lambda DNA (dam-) – 250 µg
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.
New England Biolabs, Inc. PhiX174 Virion DNA – 50 µg
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 10^6 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.
New England Biolabs, Inc. pBR322 Vector – 50 µg
pBR322 DNA is a commonly used plasmid cloning vector in E. coli and is a double-stranded circle 4,361* base pairs in length. pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight is 2.83 x 10^6 daltons.
Vector Biolabs Suppressor of cytokine signali
Cre Inducible (FlexON) GCaMP6s Adenovirus (Ad-DIO-GCaMP6s)
Vector Biolabs human AKT1 shRNA Adenovirus A
TBG promoter driven Cas9/CRISPR AAV AAV (AAV-TBG-saCas9)
