Molecular Biology Reagents and Kits
Zymo Research Corporation Quick-RNA MagBead (96 preps)
Total RNA is eluted into 50 l of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.
Active Motif RNA Subcellular Isolation Kit
Active Motif's RNA Subcellular Isolation Kit provides an efficient method to isolate nuclear and cytoplasmic RNA without cross-contamination. It can be used to isolate both short and long RNA sequences (e.g. miRNA, piRNA, snoRNA, mRNA, and lncRNA). RNA molecules exist at different levels of maturation and processing within a cell at any given time. Such heterogeneity can bias downstream analysis and interpretation of data. By isolating RNA from specific subcellular fractions, the accuracy of gene expression analysis can be greatly improved. Subcellular isolation enhances the ability to detect low abundance transcripts and aids in the ability to study RNA processing dynamics by reducing the background from other intronic or mature RNAs. Additionally, subcellular isolation helps […] For more information, please visit: https://www.activemotif.com/catalog/1160.
Seracare Life Sciences Inc Seraseq TNA (DNA/RNA) WT Mix | 2 x 20 µl
Seracare has developed two formats of total nucleic acid (TNA) derived from GM24385 human cell line as negative sample reference materials for use in targeted NGS assays. The cell line is applied as the WT background in all existing Seraseq tumor DNA and RNA products. As a negative reference material, they have extracted DNA and RNA from GM24385 as a buffered mix (TNA WT) as well as a formalin fixed and paraffin embedded option (FFPE curls) to support labs validating assay specificity at wildtype base positions, as well as specificity at positions affected by mutations included in all of the Seraseq RNA and DNA (Mutation Mix and Reference Material) products.
Seracare Life Sciences Inc Seraseq ctDNA Mutation Mix v2 WT | 1 x 25 µL
To overcome the lack of patient-like reference materials for liquid biopsy, including drawbacks of existing methodologies such as sonication, SeraCare has developed a breakthrough technology that produces the most patient-like materials compared to native ctDNA. The Seraseq ctDNA Mutation Mix v2 is a highly multiplexed product for NGS-based ctDNA assays targeting cancer-relevant somatic mutations. This specific ctDNA Mutation Mix v2 product it the wild-type with an allele frequency of 0%.
Macherey-Nagel NucleoSpin RNA XS, 50 preps
NucleoSpin RNA XS (50) 50 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
Zymo Research Corporation RNA Clean & Concentrator MagBead (96 preps)
The RNA Clean & Concentrator Magbead kit provides a simple and reliable high-throughput method for the rapid preparation of high-quality, DNA-free (R1082) RNA. This simple procedure is based on the use of magnetic-bead based technology and a unique single-buffer system that allows for recovery of total RNA (> 17 nt) from any RNA sample. The procedure is easy: Add binding buffer and MagBinding Beads to your sample, then bind, wash and elute ultra-pure RNA. The highly concentrated, purified RNA is suitable for Next-Gen sequencing, RT-qPCR and other downstream applications.
Ubpbio IPTG, 10G
Protein Purification Reagents – IPTG (UBPBio catalog# P1010-10, P1010-25, P1010-100) – IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in E. coli. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.