DNA Vectors
SignaGen Laboratories AAV-Syn-FLPo (AAV Serotype 5) | 1 x >30 µL
FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.
Agilent Technologies PBLUESCRIPT2SK(+)PHAGEMIDKIT
pBluescript II SK( + ) Phagemid Kit 1 kit f1 origin in ( + ) orientation Sac--> Kpn polylinker orientation Contains: 20microgram pBluescript II SK( + ) phagemid vector Host Strain: XL1-Blue MRF
ANTIBODY DESIGN LABS CM13d3
CM13d3 is a helper phage engineered for multivalent phage display. CM13d3 is a derivative of M13KO7 (1) having a wild-type pIII phenotype which allows efficient bacterial transduction but lacking a functional pIII gene thus driving the display of pIII-fusion proteins expressed by the phagemid on the phage head (2). The use of pIII-defective helper phage has been shown to increase display of scFv by a factor up to 100-fold and more (3-5). This preparation contains enough helper phage to superinfect up to 0.5 L (500 ml) of TG1 culture. Other bacterial strains may require different amounts of CM13d3 helper phage.
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
Abcam pCambia2300 Plant Expression Vector
pCambia2300 Plant Expression Vector
The product is subject to the following: Abcam Restricted Use Statement
Abcam pCambia1300 Plant Expression Vector
pCambia1300 Plant Expression Vector
The product is subject to the following: Abcam Restricted Use Statement
AB Vector LLC GC (Green Control), 1 mL
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks.
Agilent Technologies PSHUTTLE-CMV VECTOR
pShuttle-CMV Vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb).
TRIBIOSCIENCE INC Universal AAV qPCR Titer Kit, 100 Tests
AAV qPCR FAST Titer Kit incorporates TribioScience FAST technology paradigm and Probe qPCR technology for an accurate, one-step, and time-saving qPCR titration of AAV serotypes. The probe is labeled with Fluorescence FAM.
Sigma Aldrich Fine Chemicals Biosciences CRISPR LacZ Positive Contr1EA
Recent publications using CRISPR/Cas9-mediated recombineering in E. coli tout editing efficiencies near 100% making CRISPR/Cas9-mediated recombineering the most powerful bacterial genome engineering method to date. In addition Cas9-mediated recombineering overcomes the dependence on a second recombination step avoids the creation of destabilizing scar sites can be used in multiplexing and is less time-consuming than previous protocols.Here we present a novel dual-vector CRISPR/Cas-mediated»-Red system for improved recombineering in E. coli. Our system is shown to facilitate homology-directed repair of DSBs created by Cas9 endonuclease enabling genetic alterations through chromosomal integration of a donor DNA.This plasmid is to be used in combination with the Cas9 Lambda Red homologous recombination plasmid for E. coli (CAS9BAC1P) as the positive control for your custom gene editing experiment. The custom gRNA (CRISPRBACD) can be designed and ordered through https//www.sigmaald
New England Biolabs, Inc. Lambda DNA (dam-) – 1250 µg
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.