DNA Vectors
SignaGen Laboratories AAV-Syn-FLPo (AAV Serotype 5) | 1 x >30 µL
FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.
BIOHIPPO AAV-CaMKIIa-DIO-EGFP-5'miR-30-shRNA(scramble)-3'miR-30-WPREs
Recombinant adeno associated virus expressing 5'miR-30-shRNA(scramble)-3'miR-30 fused to EGFP under the CaMKIIa promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
AB Vector LLC GC (Green Control), 1 mL
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks.
IBA LifeSciences StrGate Acptr Vctr pESG-IBA103
The pESG-IBA103 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with a C-terminal Twin-Strep-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
Apexbio Technology LLC Safe DNA Gel Stain 100x1ml
Safe DNA Gel Stain is a fluorescent nucleic acid dye developed for visual detection of DNA or RNA fragments in agarose or polyacrylamide gels during electrophoresis procedures Structurally this reagent binds nucleic acids by intercalating into their double-stranded regions resulting in enhanced fluorescence upon excitation by ultraviolet (UV) or blue light transilluminators It is commonly utilized in molecular biology workflows such as agarose gel electrophoresis polyacrylamide gel electrophoresis and nucleic acid gel purification Designed as a safer alternative to ethidium bromide it reduces mutagenic risks associated with DNA staining and UV exposure thus facilitating downstream recombinant and cloning experiments Safe DNA Gel Stain is provided as a concentrated solution compatible with DNA and RNA visualization in routine laboratory gel-based analyses
BIOHIPPO AAV-hSyn-NES-His-CaMPARI2-L398T-Flag-HA-Myc-WPRE-SV40-polyA
Recombinant adeno associated virus expressing CaMPARI2(L398T) Flag HA Myc NES-His under the hSyn promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.