DNA Extraction and Purification
Zymo Research Corporation ZR BashingBead™ Lysis Tubes (2 mm) (50 tubes)
Each impact resistant 2 ml tube contains 0.7 ml dry volume 2.0 mm ZR BashingBead™ lysis matrix. The state of the art, ultra-high density beads are fracture resistant, chemically inert, and ideal for disrupting tough-to-lyse biological samples. These beads are ideal for tissues, insects, plant material, etc.
Zymo Research Corporation Quick DNA Fecal/Soil Microbe MiniPrep™ Kit (50 Preps)
The kit can be used to successfully isolate DNA from tough-to-lyse Gram-positive and Gram-negative bacteria, fungi, algae, protozoa, etc. that inhabit fecal and soil samples. The procedure is easy and can be completed in as little as 15 minutes: fecal samples (150 mg each) or soil samples (250 mg each) are added directly to a ZR BashingBead™ Lysis Tube (0.1 & 0.5 mm) and rapidly and efficiently lysed by bead beating without the use of organic denaturants or proteinases. Zymo-Spin™ Technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that inhibit PCR. The DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, etc.
Zymo Research Corporation ZymoBIOMICS MagBead (4 x 96 preps)
The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, fecal, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Zymo Research Corporation Universal Methylated Human DNA Standard& Control Primers (Human)
The primer set is designed to amplify a fragment of the human MLH1 mismatch repair gene following bisulfite treatment. The methylated cytosines comprising CG dinucleotides remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted into uracil and detected as thymine after PCR.
Zymo Research Corporation ssDNA/RNA Clean & Concentrator™ (50 Preps)
This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin™ Column technology. Single stranded DNA or RNA (17 to 200 nucleotides; e.g., short transcripts, probes, primers) can be safely treated and recovered using this kit. The result is highlyconcentrated ( 6 l), purified DNA/RNA that is suitable for subsequent molecular methods including PCR, RT/PCR, hybridization, etc.
Zymo Research Corporation Oligo Clean & Concentrator (50 Preps)
There is no need for organic denaturants or chloroform. Instead, the kit features Zymo-Spin column technology and employs a single-buffer system that allows for efficient oligonucleotide adsorption to the matrix of Zymo-Spin™ IC Column. Oligonucleotide is washed and concentrated into a small volume of water ( 6 l). Purified oligonucleotide, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Zymo Research Corporation ZR BAC DNA Miniprep Kit, 25 Preps
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
Zymo Research Corporation DNA Clean & Concentrator™-100 (50 Preps) w/ Zymo Spin V
The DCC-100 employs a single-buffer systemthat allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ V Columns. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ V Column with Reservoir. There is no need for organic denaturants or chloroform. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a centrifuge or vacuum source together with a microcentrifuge.
Zymo Research Corporation DNA Clean & Concentrator™-100 (25 Preps) w/ Zymo Spin V
The DCC-100 employs a single-buffer systemthat allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ V Columns. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ V Column with Reservoir. There is no need for organic denaturants or chloroform. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a centrifuge or vacuum source together with a microcentrifuge.
Zymo Research Corporation EZ DNA Methylation-Gold™ Kit (200 Rxns)
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Genomic DNA Clean & Concentrator™ Kit (100 Preps)
There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied ZymoSpin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Zymo Research Corporation Zyppy™ Plasmid Miniprep Kit (50 Preps)
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (200 Rxns)
The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Genomic DNA Clean & Concentrator™ Kit-10 (25 Preps)
There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied ZymoSpin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.