DNA Extraction and Purification
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (800 Preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l).In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation Quick-DNA™ Miniprep Plus Kit (200 Preps)
The Quick-DNA™ Miniprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid,cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin™ Technology allow for ultra-pure and concentrated genomic DNA 50 kb to be eluted in as little as 35 l. Zymo-Spin™ Columns ensure no buffer retention. Purified DNA is RNA-free, bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Biotium RNase-Free Calf Thymus DNA, 1 mg/mL (1 mL)
During isolation of DNA, RNase treatment is performed to eliminate contaminating RNA. Because RNase activity is robust and resistant to denaturation, commercial preparations of DNA contain high levels of residual RNase activity, even after re-purification. Preparing DNA solutions in DEPC-treated water will not inactivate RNase already present in the DNA. Similarly, commercial RNase inhibitor additives are usually poorly effective, expensive, and interfere in downstream applications. Biotium has developed a unique proprietary method that irreversibly inactivates RNases in purified DNA. The resulting DNA solution is free of excess protein or other chemicals that may interfere with downstream applications. Our RNase-free Calf Thymus DNA solution undergoes stringent quality control testing to confirm the product is 100% free of RNase activity. The solution is provided as 1 mg/mL calf thymus DNA in TE. Store the product at 4⁰C.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (10 Preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l).In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation Quick DNA Universal Microprep Kit (50 Preps)
For high yield total DNA extraction (e.g., genomic, mitochondrial, viral), cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 l. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Zymo Research Corporation ZymoPURE™ Wash 1 (20 ml)
ZymoPURE™ Wash 1 (20 ml).Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (50 Preps)
The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation ZymoPURE P3 (Yellow), 150 ml
ZymoPURE P3 (Yellow) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Zymo Research Corporation ZymoPURE Wash 2 (Concentrate), 28 ml
ZymoPURE™ Wash 2 Concentrate (28 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
Teknova Diluent Solution. 1000mL, Sterile.
1.00% Bovine Serum Albumin(BSA), 0.05% (v/v) Tween-20, 135mM Sodium Chloride,2.7mM Potassium Chloride,4.3mM Disodium Phosphate,1.4mM Monopotassium Phosphate
Zymo Research Corporation DNA Clean & Concentrator™-5 (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
The DNA Clean & Concentrator-5 (DCC-5) can also be used for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield DNA that is free of salts and contaminants in just 2 minutes. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.