DNA Extraction and Purification
101 BIO Endotoxin-Free Plasmid Miniprep Kit
Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed. 3 ~30 μg transfection-grade plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit within 30 minutes. The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.
Biotium DNA Gel Extraction Kit - 50 Columns
Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify DNA fragments from agarose gels. The kit has a maximum binding capacity of 10 ug DNA per column and can be used to purify DNA fragments from 40 bp to 40 kb. The kit is suitable for purifying DNA from agarose gels made with TAE or TBE buffer. The DNA Gel Extraction Kit removes GelRed and GelGreen Nucleic Acid Gel Stains, along with other commonly used nucleic acid gel stains. The purified DNA is compatible with common downstream applications such as transformation, sequencing, PCR, and restriction digests.
Supply Solutions Roche - RNase, DNase-free; from bovine pancreas; pkg of 500 ug (1 ml)
Roche - RNase, DNase-free; from bovine pancreas; pkg of 500 ug (1 ml)
Supply Solutions Roche - Biotin-16-dUTP, Solution; pkg of 50 uL (50 nmol; 1mM)
Roche - Biotin-16-dUTP, Solution; pkg of 50 uL (50 nmol; 1mM)
New England Biolabs, Inc. RNase H – 1250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
Boca Scientific Inc GeneFix 1ml DNA/RNA Saliva Collectors, Pack of 50
GeneFix is an easy-to-use saliva collection device that stabilizes DNA and viral RNA in tubes pre-filled with a non-toxic stabilization buffer. GFX-01 is developed for 1ml of saliva. The collector has a simple screw-on funnel for saliva delivery directly into the buffer. The saliva collection tube clearly indicates the volume of saliva required. Once in the buffer, DNA is stabilized for 5 years and viral RNA is stabilized for 5 days at room temperature, with high yields maintained. With leakproof collection tubes, GeneFix saliva collectors are fully specified for sample transport and storage.
Zymo Research Corporation ZymoPURE™ P2 (Green) (13 ml)
ZymoPURE P2 (Blue) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Norgen Biotek Corp Genomic DNA Isolation Kit, 100 Prep
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
Takara Bio NucleoBond Xtra Maxi EF, 50 preps
NucleoBond Xtra Maxi EF 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A.
Zymo Research Corporation Direct-zol DNA Miniprep Kit (50 Preps.)
Upon lysis of the sample with TRIzol or similar, RNA and DNA is bound directly to the Zymo-Spin™ Column. Then simply spin, wash, and elute high-quality RNA and DNA into separate fractions. No phase separation, precipitation, or post purification steps are necessary. The eluted nucleic acids are suitable for all subsequent molecular manipulations and analyses including Next-Gen sequencing, RT/qPCR, hybridization, etc.
Zymo Research Corporation MagBinding Beads, 16 ml
MagBinding Beads for use with Zymo Research magnetic bead-based purification kits. Sold separately or as part of a complete magnetic bead purification kit.
Zymo Research Corporation Quick-DNA™ Plus 96 Magbead Kit (4 x 96 Preps)
The Quick-DNA™ MagBead Plus Kit is the innovative reagents and Zymo Researchs unique system allow for a simple Bind, Wash, & Elute procedure that is unmatched in providing ultrapure and high yielding DNA (up to 150 kb). Isolated DNA is ready for immediate use in sensitive downstream applications including DNA sequencing (NGS and long read), qPCR, arrays, and methylation analysis.
