RNA Preparation and Purification
Norgen Biotek Corp Urne Cellfree Circ Rna Minikit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 250 µL to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A))(Synonyms: HyperScribe™ mRNA Kit, HyperScribe™ ARCA Kit, T7 ARCA-poly(A) mRNA Kit, All-in-One mRNA Synthesis Kit), 25 rxns.
HyperScribe All in One mRNA Synthesis Kit (ARCA T7 poly(A)) is designed for rapid in vitro generation of capped and polyadenylated mRNA transcripts Utilizing T7 RNA polymerase this system incorporates Anti-Reverse Cap Analog (ARCA) a Cap0 analog co-transcriptionally at the 5 terminus to confer mRNA stability and enhance translational efficiency After template DNA removal via DNase I treatment Poly(A) polymerase adds a poly(A) tail at the 3 -end further stabilizing the transcript and increasing translation initiation efficiency This kit is employed in research involving translation systems antisense RNA RNA interference RNA vaccines structural RNA studies and ribonuclease assays
Zymo Research Corporation Direct-zol™ RNA PreWash (Concentrate) (160 ml)
Direct-zol™ RNA Pre-wash (160 ml) for use with Direct-zol™ RNA Purification Kits for rapid isolation of high-quality total RNA including small RNAs (>17 nt) from samples in TRI-Reagent, TRIzol or similar.
Zymo Research Corporation Load N Go Quick-DNA/RNA HT Kit
The Load N' Go Quick-DNA/RNA HT kit offers a streamlined solution for high-throughput nucleic acid extraction from a variety of biological and clinical samples, such as swabs, biological liquids, and tissues. Compatible with robotic sample processors, this kit ensures the efficient isolation of high-quality DNA and RNA, making it suitable for sensitive downstream applications, including Next-Gen sequencing and RT/qPCR, ultimately enhancing the precision and reliability of scientific research.
Research Products International Corp Quick-RNA MiniPrep Kit w/Zymo-Spin IIICG Columns, Capped, & Spin-Away Filters, 50 Preps
The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
Zymo Research Corporation Quick-RNA™ MicroPrep Kit (200 Preps) w/Zymo-Spin™ IC Columns (Capped)
The Quick-RNA Microprep Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin™Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Cellscript Inc ScriptCap™ m⁷G Capping System, 25 reactions, Post-transcriptional 5' capping, ~100% Cap 0 capping efficiency
ScriptCap™ m⁷G Capping System, 25 reactions, Post-transcriptional 5' capping, ~100% Cap 0 capping efficiency
Zymo Research Corporation Quick-DNA/RNA HT
TheQuick-DNA/RNAHTkit is intended for rapid, high-throughput nucleic acid extraction (automated or manual) from any biological or clinical sample (e.g., swabs – nasal/nasopharyngeal, oropharyngeal, etc.; biological liquids – blood, plasma, serum, saliva, sputum, cells in suspension, etc.; tissue – needle biopsies, LCM, etc.) and/or samples stored in most collection matrices and devices (e.g., UTM, VTM, *DNA/RNA Shield™, RNAlater, RNAProtect, etc.). The kit is compatible with robotic-type sample processors (i.e, bead movers) in combination with sensitive downstream molecular amplification assays. High-quality DNA/RNA extracted with theQuick-DNA/RNAHTkit can be used for Next-Gen sequencing, RT/qPCR and more.
Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)), 25rxns
HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)) is designed for quick production of ARCA capped, 5mCTP and UTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) / UTP (Catalog No. B7972) can reduce host cell immune response. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. The kit contains sufficient reagents to carry out 25 reactions, 20 l each time. Up to 25-180 g of RNA can be generated with 1 g of control template per standard reaction.
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 96 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 24 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life