RNA Preparation and Purification
New England Biolabs, Inc. NEBNext RNA Depletion Core Reagent Set - 24 rxns
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool. The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. The kit is also available with RNAClean beads.
Macherey-Nagel NucleoSpin RNA Clean-up, 250 preps
NucleoSpin RNA Clean-up (250) 250 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers
Zymo Research Corporation Load N Go Quick-DNA/RNA HT Kit
The Load N' Go Quick-DNA/RNA HT kit offers a streamlined solution for high-throughput nucleic acid extraction from a variety of biological and clinical samples, such as swabs, biological liquids, and tissues. Compatible with robotic sample processors, this kit ensures the efficient isolation of high-quality DNA and RNA, making it suitable for sensitive downstream applications, including Next-Gen sequencing and RT/qPCR, ultimately enhancing the precision and reliability of scientific research.
Norgen Biotek Corp Urne Cellfree Circ Rna Minikit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 250 µL to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Norgen Biotek Corp Plasma/Serum RNA Purification Midi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. Plasma samples prepared from blood collected on heparin should not be used.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids.The purified plasma/serum RNA is fully compatible with all downstream applications including NGS and more
Norgen Biotek Corp Total RNA Purification 96-Well Kit
Extract high quality & purity total RNA including miRNA . Convenient high throughput format for vacuum, centrifuge or robot. Very consistent well-to-well RNA isolation. No phenol step required - isolate all RNA in one fraction. Bind & elute all RNA irrespective of size or GC content, without bias. Isolate from a wide variety of specimens.
Norgen Biotek Corp FFPE RNA Purification Kit
Norgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time.
Omega Biotek Inc 2 mL Collection Tubes, 5000/cs
2.0 mL Tube, Capless, graduated. 100/pack, 50 pack/case.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (10 ug) - 100 preps
The Monarch Spin RNA Cleanup Kit (10 ug) rapidly and reliably purifies up to 10 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. The kit can also be applied for total RNA extraction from some sample types. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 50 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Research Products International Corp ZR-96 RNA Clean & Concentrator Kit, 2 x 96 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.