RNA Preparation and Purification
RNA Preparation and Purification
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (200 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.
Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
![Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-R2051DIRECTZOLRNAMINIPREPYES.JPG-250.jpg)
Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Macherey-Nagel NucleoSpin RNA Clean-up, 250 preps
NucleoSpin RNA Clean-up (250) 250 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers
![Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502037264.JPG-250.jpg)
Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]
The extraction method inactivates viruses and other infectious agents. Total RNA (including small/microRNAs) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, DNA/RNA Shield™ samples, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRIzol/TRI Reagent, wash and elute the RNA. No chloroform, phase separation or precipitation steps are necessary. High-quality total RNA is ready for Next-Gen sequencing, RT-qPCR, transcription profiling, hybridization, etc
New England Biolabs, Inc. NEBNext Ultra™ II RNA Library Prep Kit for Illumina – 96 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
New England Biolabs, Inc. NEBNext Ultra™ II RNA Library Prep Kit for Illumina – 24 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
New England Biolabs, Inc. Monarch RNA Cleanup Kit (500 µg) – 10 preps
The Monarch RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience.
Sigma Aldrich Fine Chemicals Biosciences RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
Molecular Research Center RNAZOL RT REAGENT 200ML
A single-step reagent isolating mRNA and small RNA in separate fractions. No chloroform-induced phase separation or refrigerated centrifuge required. No DNase treatment. Higher yields and quality than previous single-step methods.
New England Biolabs, Inc. Monarch RNA Cleanup Kit (50 µg) – 10 preps
The Monarch RNA Cleanup Kit (50 ug) rapidly and reliably purifies up to 50 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in downstream applications including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch RNA Cleanup kits are also available for 10 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience.
New England Biolabs, Inc. Monarch RNA Cleanup Kit (500 µg) – 100 preps
The Monarch RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience.
Omega Biotek Inc NGS Workflow PCR Clean Up Mag Bind Total Pure NGS
The Mag-Bind TotalPure NGS kit efficiently purifies PCR products for next-generation sequencing, removing impurities with high recovery rates. It includes all necessary components for processing 5-100μL PCR volumes and is compatible with major sequencing platforms.
BIOSEARCH TECHNOLOGIES INC (Lucigen) NxGen RNAse Inhibitor, 10,000 U @ 40 U/LEliminate RNase activity.
RNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.Supplied at 40,000 U/mL in 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol, pH 7.5 @ 25¿C.
Norgen Biotek Corp RNase-Free DNase I Kit, 50 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.