RNA Preparation and Purification
Zymo Research Corporation Quick-RNA MagBead (96 preps)
Total RNA is eluted into 50 l of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.
Molecular Research Center RNAzol RT, 500 mL
RNAzol RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.
Molecular Research Center RNAZOL RT REAGENT 200ML
A single-step reagent isolating mRNA and small RNA in separate fractions. No chloroform-induced phase separation or refrigerated centrifuge required. No DNase treatment. Higher yields and quality than previous single-step methods.
Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
Macherey-Nagel NucleoSpin RNA Plus XS, 50 preps
NucleoSpin RNA Plus XS (50) 50 preps for the isolation of total RNA - NucleoSpin RNA Plus XS Columns, NucleoSpin gDNA Removal XS Columns, Collection Tubes, buffers
Norgen Biotek Corp Animal Tissue RNA Purification Kit
This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, and intestine. The tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, NGS and more.The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
(Lucigen) NxGen RNAse Inhibitor, 10,000 U @ 40 U/LEliminate RNase activity.
RNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.Supplied at 40,000 U/mL in 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol, pH 7.5 @ 25¿C.
Omega Biotek Inc NGS Workflow PCR Clean Up Mag Bind Total Pure NGS
The Mag-Bind TotalPure NGS kit efficiently purifies PCR products for next-generation sequencing, removing impurities with high recovery rates. It includes all necessary components for processing 5-100μL PCR volumes and is compatible with major sequencing platforms.
Biochain Institute Inc Total RNA - Lupus: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Monkey (Rhesus) Normal Tissue: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Ultra™ II RNA First Strand Synthesis Module – 96 reactions
This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1 g total RNA. The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming. The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1µg total RNA.