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The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis. This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 250 ng and as high as 5000 ng. The higher yield results from an improved cDNA purification system, now provided with the kit. The Single-Round RNA Amplification and Biotin Labeling System has been optimized for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and purification and in vitro transcription labeling. Note: Control RNA (100µg/ml) (ENZ-42406-CR) is available separately.
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The Enzo Life Sciences' 100-Reaction Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples (1µg-5µg) in less than 24 hours for array analysis. While cDNA quantities produced are sufficient for gene expression, manual sample preparation involving multiple enzymatic reactions and product purification steps are time consuming and error-prone. The 100-Reaction Single- Round RNA Amplification and Biotin Labeling System has been optimized for superior performance across several automated liquid handling platforms. The result is a comprehensive system that reduces variability while subsequently improving reproducibility, data quality and throughput for automated genomic environments. The complete system is composed of reagents for cDNA synthesis and in vitro transcription labeling and does not provide materials required for purification.
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5-Propargylamino-dCTP is a modified nucleoside triphosphate analog featuring a cytosine base with a propargylamino substituent introducing an alkyne functional group 5-Propargylamino-dCTP is incorporated into DNA strands mediated by DNA polymerases during active DNA synthesis This modification enables covalent conjugation to azide-bearing probes via copper-catalyzed azide-alkyne cycloaddition (CuAAC) Based on these pharmacological properties 5-Propargylamino-dCTP holds research potential in the detection and analysis of cellular proliferation DNA replication dynamics nucleic acid repair subcellular localization studies fluorescence labeling and molecular imaging-based nucleic acid research methodologies
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