Miscellaneous Molecular Biology Reagents
Invitrogen™ Yeast RNA (5 mg/mL)
Purified Saccharomyces cerevisiae yeast RNA is is a very effective blocking agent when used in Northern prehybridization and hybridization buffers, as well as being suitable as a coprecipitant in nucleic acid precipitations.
Thermo Scientific™ DNA Loading Dye & SDS Solution (6X)
Perform agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins with this DNA loading dye containing SDS.
Invitrogen™ ATP Solution (10 mM)
This Invitrogen ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript™ Kits and is supplied in one tube containing 100 μL.
Thermo Scientific™ 5-Fluoroorotic Acid
Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.
Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution
UltraPure Guanidine Isothiocyanate Solution consists of 4M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA.
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
Invitrogen™ UltraPure™ Sucrose
UltraPure Sucrose is suitable for forming density gradients for a variety of separation applications.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Invitrogen™ IPTG
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
Invitrogen™ UltraPure™ Formamide
UltraPure Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization.
Invitrogen™ UltraPure™ Cesium Chloride, Optical Grade
UltraPure™ Cesium Chloride, Optical Grade is specifically prepared for use in density gradients.
Thermo Scientific™ Co-factors
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Thermo Scientific™ X-Gal
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ Agarose II (Low Melt)
Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.