RNA Controls
Apexbio Technology LLC EZ Cap Cy5 EGFP mRNA (5-moUTP) 1mg (1mg/mL)
EZ Cap Cy5 EGFP mRNA (5-moUTP) expresses enhanced green fluorescent protein (EGFP) which emits green fluorescence observable at 509 nm It is suitable for assays such as mRNA delivery translation efficiency cell viability and in vivo imaging This product is provided at 1 mg/ml It is co-transcriptionally capped with a Cap 1 structure incorporated 5-moUTP (5-Methoxy-UTP) and a poly(A) tail Cy5 is a synthetic red fluorescent dye with maximum excitation and emission wavelengths at approximately 650 nm and 670 nm respectively During transcription Cy5-UTP and 5-moUTP are incorporated at a ratio of 1 3 allowing for easy visualization of mRNA while maintaining translation capability in cell culture Generally translation efficiency inversely correlates with Cy5-UTP substitution level This product is designed to mimic fully processed and mature mRNA It is an ideal product for investigating mRNA delivery localization translation and other behaviors
Apexbio Technology LLC EZ Cap EGFP mRNA (m1Ψ) 5x1mg (1mg/mL)
Enhanced green fluorescent protein (EGFP) is a commonly used reporter molecule that emits intense and bright green fluorescence upon excitation EZ CapTM EGFP mRNA (m1 ) is provided at a concentration of approximately 1 mg/mL It features a Cap 1 structure generated through co-transcriptional capping which is more suitable for mammalian systems and offers higher transcription efficiency compared to Cap 0 structures (ARCA and mCap) Incorporation of N1-Methylpseudo-UTP (m1 ) and a poly (A) tail helps suppress RNA-mediated innate immune activation and enhances mRNA stability and lifespan both in vitro and in vivo Additionally the poly (A) tail plays a crucial role in enhancing the efficiency of translation initiation
Apexbio Technology LLC Single Firefly Luciferase Reporter Gene Assay Kit 1000T
The luciferase-luciferin bioluminescent system provides a highly sensitive and efficient tool for detecting gene expression In the presence of ATP oxygen and magnesium ions firefly luciferase catalyzes the oxidation reaction of luciferin into oxyluciferin accompanied by the emission of bioluminescence at about 560 nm This bioluminescence signal can be detected using a luminometer or liquid scintillation counter The Single Firefly Luciferase Reporter Gene Assay Kit allows direct quantification of intracellular firefly luciferase activity via chemiluminescence without the need for washing or harvesting cells This assay is a one-step detection kit providing exceptional sensitivity high signal stability and ease of use The luciferase detection reagent included in this kit can be directly added to cultured cells at equal volumes to the medium This assay is also suitable for measurements of previously lysed or stored cell samples
Integrated DNA Technologies Inc Alt-R CRISPR tracrRNA, 5 nmol
Universal 67mer tracrRNA that contains proprietary chemical modifications conferring increased nuclease resistance. Hybridizes to crRNA to activate the Cas9 enzyme.
Invivogen 5'ppp-dsRNA Control / LyoVec, 25 ug
5’ppp-dsRNA Control is a negative control for 5’ triphosphate double-stranded RNA (5’ppp-dsRNA), a synthetic ligand for the retinoic acid-inducible protein I (RIG-I, also known as Ddx58). RIG-I is a cytoplasmic RNA helicase that belongs to the RIG-I-like receptors (RLRs) family and triggers an antiviral immune response by the activation of type-I interferons (IFN-alpha and -beta).
Sigma Aldrich Fine Chemicals Biosciences Erythropoietin EPO human r
Recombinant human erythropoietin (EPO) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of ~18 kDa. This protein is manufactured in human cells using an all-human production system with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding and often supports better stability of the protein in culture. The protein is produced with no artificial tags.
Apexbio Technology LLC ARCA Cy5 EGFP mRNA (5-moUTP) 5x1mg (1mg/mL)
ARCA Cy5 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency Upon transfection of cells with ARCA Cy5 EGFP mRNA (5-moUTP) EGFP (Enhanced Green Fluorescent Protein) is expressed EGFP is a frequently used reporter protein that emits strong and bright green fluorescence upon excitation with an excitation/emission maximum of 488 nm/509 nm respectively This mRNA is labeled with the fluorescent dye Cyanine 5 (Cy5) allowing direct visualization of EGFP mRNA upon excitation at specific wavelengths the excitation and emission maxima for Cy5 are 650 nm and 670 nm respectively Cy5 EGFP mRNA is thus ideally suited for analyzing mRNA delivery and localization independently from translation This product is a synthetic mRNA modified by adding a cap structure at the 5 end (Cap) a poly(A) tail at the 3 end and inclusion of the modified nucleotides 5-moUTP and Cyanine 5-UTP The incorporation ratio of Cyanine 5-UTP to 5-moUTP is 1 3 The cap st
Apexbio Technology LLC EZ Cap CM-EGFP Probe mRNA (m1Ψ) 1mg (1mg/mL)
CM-EGFP mRNA encodes a fusion protein comprised of a cell membrane (CM) targeting signal sequence and enhanced green fluorescent protein (EGFP) By fusing EGFP with a membrane localization signal this probe enables real-time visualization of dynamic changes occurring at the cell membrane of living cells particularly beneficial for research involving cell membrane-related processes such as signal transduction membrane protein interactions and alterations in membrane structure EZ Cap CM-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL It is specifically designed for cell membrane-specific expression of a green fluorescent protein label This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
Amsbio LLC Total RNA - Human Adult Normal Tissue: Bone Marrow
Total RNA - Human Adult Normal Tissue: Bone Marrow
Amsbio LLC Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte
Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte