Competent Cells
New England Biolabs, Inc. NEB® 10-beta Electrocompetent E.coli – 6 x 0.1 ml/tube
NEB 10-beta electrocompetent E. coli cells are optimized for high efficiency transformation by electroporation. These cells are ideal for DNA library constructions and all cloning purposes.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium includedFree of animal products
New England Biolabs, Inc. NEBExpress Iq Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
High efficiency chemically competent E. coli cells suitable for non T7 expression with enhanced reduction of basal expression.
New England Biolabs, Inc. NEB® 10-beta Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni 10G ELITE Electrocompetent Cells,24 Rxns (SixPacks)Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies.
Lucigens E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability, while decreasing costs. These cells provide solutions for a wide range of applications at economical prices. E. cloni 10G & 10GF ELITE Electrocompetent Cells > 2 10^10 cfu/g pUC DNA. ELITE Cells have twice the transformation efficiency compared to ultra high efficiency cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
INTACT GENOMICS INC DirectPlate TG1 Phage Display Chemically Competent Cells
Intact Genomics (ig) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ TG1 Phage Display chemically competent E. coli cells provide higher transformation efficiency than any competitor’s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, phage display libraries and sub-cloning.Product Includes and Storage:DirectPlate™ TG1 Phage Display competent cells: -80 ºCpUC19 control DNA: -20 ºC
New England Biolabs, Inc. NEB® 5-alpha Competent E. coli (High Efficiency) – 96 x 50 µl/tube (12 x 8-tube strips)
NEB 5-alpha Competent E. coli (High Efficiency) is a derivative of the popular DH5. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Perkin Elmer US LLC pergo 2 Humidifier without Nebulizer
This assembly includes: pergo main unit (heater), Qty. 1 PFA water reservoir with min/max lines and four ported caps, Qty. 1 pergo spare power supply, 12V, 1.5A, wall mount with plugs for North America, Europe, UK, Qty. 1 2.4 mm I.D. x 4mm O.D. PTFE gas line for MicroFlow nebulizer, 1 m, Qty. 4 Gas fitting, Pkg. 10 Note: This assembly does not include a nebulizer.
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni 10G ELITE Electrocompetent Cells,48 Rxns (SixPacks)Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies
Lucigens E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability, while decreasing costs. These cells provide solutions for a wide range of applications at economical prices. E. cloni 10G & 10GF ELITE Electrocompetent Cells > 2 10^10 cfu/g pUC DNA. ELITE Cells have twice the transformation efficiency compared to ultra high efficiency cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
Zymo Research Corporation Mix & Go! Competent Cells-XJb(DE3) Autolysis™ (10 x 100 μl) (Add 1 ml 500x Arabinose)
Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights Simple and controlled autolysis of E. coli Strains can by lysed in minutes after harvesting Method compatible with most buffer systems Ideal for protein expression and purification, also applicable for extraction of nucleic acids Scale up to lyse more samples without increase in time or labor
Zymo Research Corporation Mix & Go! Competent Cells-HB101, 10 x 100 µl
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/µg of pUC19 DNA , which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 µl/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 µl/tube.
BIOSEARCH TECHNOLOGIES INC (Lucigen) OverExpress C41(DE3) pLysS Chem Comp Cells, 12 Rxns (SOLOs)
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
Agilent Technologies BL21 COMPETENT CELLS
BL21 Competent Cells 5 x 0.2mL For extremely toxic proteins use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based systems