Competent Cells
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni EXPRESS BL21(DE3)Chemically Competent Cells,12 Rxns (DUOs)
E. cloni EXPRESS BL21(DE3)Chemically Competent Cells,12 Rxns (DUOs)Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.Best value for routine protein expressionAvailable as Electrocompetent and Chemically Competent Cells.Each E. cloni EXPRESS Kit contains: the indicated E. cloni EXPRESS Electrocompetent or Chemically Competent Cells in DUO packaging (2 transformations per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, and complete protocols.
BIOSEARCH TECHNOLOGIES INC (Lucigen) HI-Control 10G Chemically Competent Cells, 12 Rxns (SOLOs)Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor
Reduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.Obtain high protein yields: Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.Achieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA.Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
BIOSEARCH TECHNOLOGIES INC (Lucigen) MC1061 F- Electrocompetent Cells, 12 Rxns (DUOs)High efficiency F-control strain for phage display applications.
Perfect for antibody phage display or peptide phage display library creationThe highest efficiency TG1 competent cells availableYour only source for electrocompetent SS320 and ER2738 cellsAmber suppressor and non-amber suppressor lines for maximum flexibilityHigh efficiency MC1061 F- cells for cloning or control reactionsCreate larger libraries; speed discoveryBulk custom dispensing available with all of our phage display cellsCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni 5-alpha Chemically Competent Cells, 24 Rxns (DUOs)Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
Direct replacements for standard cloning strainsOptimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
BIOSEARCH TECHNOLOGIES INC (Lucigen) TransforMax EPI300-T1R Electrocompetent E. coli, 20 RxnsClone large inserts in standard plasmids, avoid T1 and T5 phage contamination, and control copy number of CopyControl vectors.
Standard cloning for large inserts and T1 phage resistanceAvoid phage contamination with tonA mutation for resistance to bacteriophages T1 and T5.Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.Generate clones with inducible copy number using Copy Control vectors.Achieve high transformation efficienciesTransforMax EPI300-T1R Electrocompetent E. coli are supplied as 10 x 100 L aliquots, with CopyControl™ Induction Solution and pUC19 Control DNA (100 pg/L).
BIOSEARCH TECHNOLOGIES INC (Lucigen) Endura Chemically Competent Cells, 12 Rxns (DUOs)Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Lucigens Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni 10G Chemically Competent Cells, 12 Rxns (DUOs)Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies
Lucigens E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of useonly common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices. E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Neuromics, Inc MEXi-293E Cells, 1.5 ml are human embryonic kidney (HEK) cells derived from the 293 cell line and used for propagation of plasmids and expression of recombinant proteins in mammalian cells.
MEXi-293E cells are human embryonic kidney (HEK) cells derived from the 293 cell line and used for propagation of plasmids and expression of recombinant proteins in mammalian cells. The cell line expresses the EBNA-1 protein for episomal replication of oriP-harboring plasmids. The cell line is adapted for growth in suspension and for use with the MEXi mammalian expression system.Further available components of the MEXi system are a variety of pDSG expression vectors (#5-52XX-001), MEXi-CM culture medium (#2-6010-010) and MEXi-TM transfection medium (#2-6011-010).
Gold Biotechnology Inc JM109 Chemically Competent E. coli Cells 10x50 μl
GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning. JM109 is a K strain bacterium that carries multiple features including the lacIqZΔM15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol as well as the hsdR17 genotype which prevents endogenous endonucleases from cleaving DNA.
Gold Biotechnology Inc HB101 Chemically Competent E. coli Cells 5x50 μl
GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.
Gold Biotechnology Inc GB5-alpha Electrocompetent E. coli Cells 5x100 μl
GoldBio’s GB5-alpha Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our GB5-alpha cells due to mutations in both endA1 and recA1.
BIOSEARCH TECHNOLOGIES INC (Lucigen) E. cloni 10G CLASSIC Electrocomp Cells, 24 Rxns (SixPacks)Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies
Lucigens E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability, while decreasing costs. These cells provide solutions for a wide range of applications at economical prices. E. cloni 10G CLASSIC Electrocompetent Cells > 5 10^9 cfu/g pUC DNA. CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.