Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.

76 – 90 of 402 results

MilliporeSigma™ Novagen™ Origami™ B (DE3) Competent Cells

Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >2 x 10⁶ cfu/μg

MilliporeSigma™ Novagen™ Tuner™ Competent Cell Set

Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture.

Pricing & Availability

MilliporeSigma™ Novagen™ HMS174 (DE3) pLysS Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >5 x 10⁶ cfu/μg

MilliporeSigma™ Novagen™ HMS174 Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >5 x 10⁶ cfu/μg

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >2 x 10⁶ cfu/μg

MilliporeSigma™ Novagen™ Rosetta™ Blue Competent Cells

RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >1 x 10⁶ cfu/μg

MilliporeSigma™ Novagen™ Rosetta™ 2 Competent Cell Set

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Pricing & Availability

MilliporeSigma™ Novagen™ NovaBlue(DE3) Competent Cells

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

Pricing & Availability
Specifications

Blue/White Screening	No
Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >1 x 10⁸ cfu/μg
Contains F' Episome	Contains F' Episome

MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells

BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >2 x 10⁷ cfu/μg

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.

Pricing & Availability

MilliporeSigma™ Novagen™ Origami™ 2 Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Pricing & Availability
Specifications

Content And Storage	≤-70°C
Efficiency	Guaranteed efficiency: >2 x 10⁶ cfu/μg

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLysS Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.