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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems.
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BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.
One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
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MultiShot BL21 Star (DE3) Chemically Competent E. coli Cells deliver enhanced mRNA stability for high-yield protein expression. The flexible 96-tube rack format supports scalable, efficient workflows for cloning and expression experiments.
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MultiShot TOP10 Chemically Competent E. coli Cells provide high-efficiency transformation for routine cloning with flexible workflow capacity. The 96-tube rack format supports scalable, high-throughput plasmid propagation and blue/white screening.
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MultiShot Mach1™ T1R Chemically Competent E. coli Cells enable rapid, high-efficiency cloning in an automation-ready 96-well format. Fast-growing and phage-resistant, these cells support same-day transformation and streamlined high-throughput workflows.
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MultiShot BL21(DE3) Chemically Competent E. coli Cells enable efficient protein expression with flexible workflow capacity. The 96-tube rack format allows convenient use with varying sample numbers for scalable, high-throughput expression and cloning experiments.
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MultiShot Stbl3 Chemically Competent E. coli Cells support cloning of unstable vectors with direct repeats. Automation-compatible 96-well format ensures high-throughput use while the Stbl3 strain minimizes recombination and maintains complex constructs.
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MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
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