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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
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BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.
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Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.
MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.1, or any other supF-containing vector).
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