Crosslinking Reagents
Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ NHS-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientific Pierce™ Pierce™ SM(PEG)2, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)2 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ NHS (N-hydroxysuccinimide)
Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.
Thermo Scientific™ DMP (dimethyl pimelimidate)
Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.
Thermo Scientific™ MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.
Thermo Scientific™ GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ KMUH (N-κ-maleimidoundecanoic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (19.0A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ EMCH (N-ε-maleimidocaproic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.
MilliporeSigma™ Calbiochem™ SMCC, Water-Soluble
Used for coupling enzymes to antibodies.
MilliporeSigma™ Calbiochem™ THIOLYTE™ Monobromobimane Reagent, Fluorescent Labeling Compound
Used to label E.coli thioredoxin.
Thermo Scientific™ BMH (bismaleimidohexane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this long crosslinker composed of maleimide groups and 11-atom (13.0A) spacer arm.