Crosslinking Reagents
Thermo Scientific™ SM(PEG)4 (PEGylated SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit
Chemically conjugate any two purified proteins (antibodies, enzymes, carriers, etc.) using sulfo-SMCC crosslinker, a complete kit with modification reagents and buffers.
Thermo Scientific Pierce™ Pierce™ SM(PEG)12, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)12 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 8-atom (9.4A) spacer arm.
Thermo Scientific™ BMPS (N-β-maleimidopropyl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short amine-to-sulfhydryl crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 5-atom spacer arm.
Thermo Scientific™ GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate))
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 12-atom (14.2A) spacer arm.
Thermo Scientific™ TMEA (tris(2-maleimidoethyl)amine)
A 3-way, sulfhydryl-to-sulfhydryl crosslinker; tris (2-maleimidoethyl) amine; composed of three maleimide groups on short spacer arms; conjugate protein or peptide cysteines.
MilliporeSigma™ Calbiochem™ SATA
Reacts with primary amines to add protected sulfhydryl groups.
Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.
Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ PDPH (3-(2-pyridyldithio)propionyl hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.
Thermo Scientific™ DMS (dimethyl suberimidate)
Crosslink amines to amines with dimethyl suberimidate (CAS 34490-86-3); composed of imidoester groups at either end of a membrane-permeable, 8-atom spacer arm.
Thermo Scientific™ BMB (1,4-bismaleimidobutane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this mid-length crosslinker composed of maleimide groups and 9-atom (10.9A) spacer arm.