Protein Labeling Reagents
Biotium Tyramide Amplification Kit with HRP Streptavidin and Biotin-XX Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP streptavidin Biotin-XX tyramide. The kit contains sufficient reagent for 50-150 slides.
Biotium MitoView™ 650
MitoView™ 650 is a far-red mitochondrial stain for live cells. Its staining is not responsive to mitochondrial membrane potential. With excitation/emission at 644/670 nm, and can be combined with visible red probes for multicolor imaging.
AdipoGen Biotin Sulfone
Chemical. CAS 40720-05-6. Formula C10H16N2O5S. Molecular Weight 276.31. Biotin sulfone is an oxidized form of biotin and is as efficient as biotin in streptavidin binding and it binds to avidin with a binding ratio of 0.332 compared with biotin. Biotinylated synthetic oligosaccharides are an attractive tool, as streptavidin coated plates and beads are commercially available and widely use in serological assays. As biotin is chemically reactive, and not compatible with carbohydrate protecting group manipulation, the use of biotin sulfone tagged oligomannosides BSTO for antigen immobilization in candidiasis or allergy diagnosis has been used. The use of biotin sulfone also offers a convenient solution for both oligosaccharide synthesis and immobilization on microspheres and surface plasmon resonance sensors.
Cayman Chemical BIotIn 4AmIdobenzoIc AcI 25mg
A biotinidase substrate; enzymatic hydrolysis releases PABA which can be quantified by colorimetric detection at 540 nm in the presence of N-1-naphthylethylenediamine as a measure of biotinidase activity; has been used in the evaluation of biotinidase deficiency
AdipoGen Fluorescein didecanoate
Chemical. CAS 7389-09-5. Formula C40H48O7. MW 640.8. Fluorescein didecanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the didecanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells. Fluorescein didecanoate is a fluorogenic substrate for C10 esterase that generates green fluorescence upon esterase cleavage. Spectral Data lambdaEx 497nM, lambdaEm 516nm. Extinction coefficient 80000 cm-1 M-1.
Cayman Chemical BIotIn-LC-LC-NHS 25mg
A biotinylation reagent; contains an NHS moiety to facilitate coupling reactions and 30.5 Å spacer to decrease steric hindrance; has been used to biotinylate small molecules such as paclitaxel and coenzyme Q10
Nanotemper Technologies Inc His-Tag Labeling Kit RED-tris-NTA 2nd Generation
Protein Labeling Kit RED-tris-NTA 2nd generation Monolith
Sigma Aldrich Fine Chemicals Biosciences Rhodamine B isothiocyanate mixed isomers, BioReagent, suitable for protein labeling | 36877-69-7 | MFCD00136007 | 500MG
Rhodamine B isothiocyanate mixed isomers, BioReagent, suitable for protein labeling | Mol Wt: 536.08 | 36877-69-7 | MFCD00136007 | 500MG
STOGGLES Square Jade Med
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AdipoGen Rhodamine 110 chloride, 13558-31-1, MFCD00042009, 1 g
MF: C20H14N2O3 . HCl, MW: 366.802, ≥97%, Appearance: Red to red-brown crystalline or powder. Fluorescent dye. Fluorophore standard that is widely used in enzyme assay systems. Precursor for a variety of R110-based enzyme substrates for flow cytometric measurements. Used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates.
AdipoGen 6-IAF,Chemical. CAS: 73264-12-7
6-IAF, AdipoGen Life Sciences. Chemical. CAS: 73264-12-7. Formula: C22H14INO6. MW: 515.25. Synthetic. Fluorescent reagent with high selectivity. 73264-12-7