Protein Modification Reagents
MilliporeSigma™ HRV 3C Protease
Highly efficient fusion tag removal at low temperature
Thermo Scientific™ MS(PEG)24 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Invitrogen™ Protease QF
Protease QF, 180 μL, is an enzyme in aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.
MilliporeSigma™ Aminopeptidase N (CD13), Human Serum
Prepared from human serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
MP Biomedicals™ Trypsin, 2.5% (w/v) Solution
Porcine pancreas-derived enzyme commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues
Thermo Scientific™ CA(PEG)4 Carboxy-PEG-Amine Compound
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
MilliporeSigma™ S·Tag Thrombin Purification Kit
Application in protein purification
MilliporeSigma™ Calbiochem™ Thrombospondin, Human Platelets
Blocks FGF-induced neovascularization of rat cornea.
MilliporeSigma™ Calbiochem™ Coenzyme A, Trilithium Salt
Serves as cofactor in enzymatic acetyl transfer reactions
MilliporeSigma™ Calbiochem™ DM-NITROPHEN™ Reagent, Tetrasodium Salt
Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis
MilliporeSigma™ Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
Thermo Scientific™ NeuCode™ Lysine-080
Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.
MilliporeSigma™ Calbiochem™ BAPTA/AM
Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA
MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction System 1
Induces protein expression without monitoring cell density.